Evidence map›Paper›PMID 33224481›Full record

ArticleF1000Research2020

Automated cell tracking using StarDist and TrackMate.

Elnaz Fazeli, Nathan H Roy, Gautier Follain, Romain F Laine, Lucas von Chamier, Pekka E Hänninen, John E Eriksson, Jean-Yves Tinevez, Guillaume Jacquemet

Open access · goldAbstract read
In one paragraph

Article in F1000Research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
8.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 65 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Tui: A Multigenerational and Expert-Correctable Tracker for Cellular Dynamics.Computational and structural biotechnology journal · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 4 countries.

Elnaz FazeliLaboratory of Biophysics, Institute of Biomedicine, Faculty of Medicine, University of Turku, Turku, Finland.ORCID 0000-0002-0770-0777
Nathan H RoyDepartment of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.
Gautier FollainTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Romain F LaineMRC-Laboratory for Molecular Cell Biology, University College London, London, UK.ORCID 0000-0002-2151-4487
Lucas von ChamierMRC-Laboratory for Molecular Cell Biology, University College London, London, UK.
Pekka E HänninenLaboratory of Biophysics, Institute of Biomedicine, Faculty of Medicine, University of Turku, Turku, Finland.
John E ErikssonTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
Jean-Yves TinevezImage Analysis Hub, Institut Pasteur, Paris, France.ORCID 0000-0002-0998-4718
Guillaume JacquemetTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.ORCID 0000-0002-9286-920X
Åbo Akademi University · FIUniversity of Turku · FIChildren's Hospital of Philadelphia · USInstitut Pasteur · FRMRC Laboratory for Molecular Cell Biology · GBThe Francis Crick Institute · GB

Funding

TRAINING PROGRAM/RHEUMATIC DISEASEST32AR007442 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI BEHRENS, EDWARD M, MERKEL, PETER A · 1986 to 2018
$5.8M
Medical Research Council MR/T027924/1NIAMS NIH HHS T32 AR007442
6 · The paper itself

Abstract

The ability of cells to migrate is a fundamental physiological process involved in embryonic development, tissue homeostasis, immune surveillance, and wound healing. Therefore, the mechanisms governing cellular locomotion have been under intense scrutiny over the last 50 years. One of the main tools of this scrutiny is live-cell quantitative imaging, where researchers image cells over time to study their migration and quantitatively analyze their dynamics by tracking them using the recorded images. Despite the availability of computational tools, manual tracking remains widely used among researchers due to the difficulty setting up robust automated cell tracking and large-scale analysis. Here we provide a detailed analysis pipeline illustrating how the deep learning network StarDist can be combined with the popular tracking software TrackMate to perform 2D automated cell tracking and provide fully quantitative readouts. Our proposed protocol is compatible with both fluorescent and widefield images. It only requires freely available and open-source software (ZeroCostDL4Mic and Fiji), and does not require any coding knowledge from the users, making it a versatile and powerful tool for the field. We demonstrate this pipeline's usability by automatically tracking cancer cells and T cells using fluorescent and brightfield images. Importantly, we provide, as supplementary information, a detailed step-by-step protocol to allow researchers to implement it with their images.

Indexed as

Cell TrackingImage Processing, Computer-AssistedCell MovementFijiSoftwareAutomated trackingCell migrationDeep-learningImage analysisStarDistTrackMate

Identifiers

PMID33224481
PMCPMC7670479
OpenAlexW3097472992

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.