Evidence map›Paper›PMID 33224460›Full record

ArticleChemical science2020

Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions.

Yan Wang, Venkatesan Kathiresan, Yaoyi Chen, Yanping Hu, Wei Jiang, Guangcan Bai, Guoquan Liu, Peter Z Qin, Xianyang Fang

Open access · diamondAbstract read
In one paragraph

Article in Chemical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
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  5. Review
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  8. Article
  9. Review
  10. Recent advances in RNA structurome.Science China. Life sciences · 2022
    Review
  11. Review
  12. Article
  13. Strategies for Covalent Labeling of Long RNAs.Chembiochem : a European journal of chemical biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Yan WangBeijing Advanced Innovation Center for Structural Biology , School of Life Sciences , Tsinghua University , Beijing 100084 , China . Email: fangxy@tsinghua.edu.cn.ORCID 0000-0002-0452-6750
Venkatesan KathiresanDepartment of Chemistry , University of Southern California , Los Angeles , California 90089 , USA . Email: pzq@usc.edu.
Yaoyi ChenBeijing Advanced Innovation Center for Structural Biology , School of Life Sciences , Tsinghua University , Beijing 100084 , China . Email: fangxy@tsinghua.edu.cn.
Yanping HuBeijing Advanced Innovation Center for Structural Biology , School of Life Sciences , Tsinghua University , Beijing 100084 , China . Email: fangxy@tsinghua.edu.cn.
Wei JiangDepartment of Chemistry , University of Southern California , Los Angeles , California 90089 , USA . Email: pzq@usc.edu.
Guangcan BaiState Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences , Peking University , Beijing 100191 , China.
Guoquan LiuState Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences , Peking University , Beijing 100191 , China.
Peter Z QinDepartment of Chemistry , University of Southern California , Los Angeles , California 90089 , USA . Email: pzq@usc.edu.
Xianyang FangBeijing Advanced Innovation Center for Structural Biology , School of Life Sciences , Tsinghua University , Beijing 100084 , China . Email: fangxy@tsinghua.edu.cn.ORCID 0000-0001-9432-9736
Beijing Advanced Sciences and Innovation Center · CNUniversity of Southern California · USPeking University · CN

Funding

Acquisition of a Pulse Electron Paramagnetic Resonance SpectrometerS10RR028992 · NCRR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI QIN, PETER Z · 2010 to 2010
$1.0M
NCRR NIH HHS S10 RR028992
6 · The paper itself

Abstract

Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance (EPR) spectroscopy has remained challenging to date. We here demonstrate an efficient and generally applicable posttranscriptional SDSL method for large RNAs using an expanded genetic alphabet containing the NaM-TPT3 unnatural base pair (UBP). An alkyne-modified TPT3 ribonucleotide triphosphate (rTPT3

Identifiers

PMID33224460
PMCPMC7667596
OpenAlexW3052585899

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.