ArticleThe New phytologist2021
The antagonistic MYB paralogs RH1 and RH2 govern anthocyanin leaf markings in Medicago truncatula.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 35 citations in OpenAlex.
- Overexpression of a Tonoplast Malate Transporter Gene Leads to Enhanced Anthocyanin Biosynthesis in Apple.Plant biotechnology journal · 2026Article
- VrMYB90 negatively regulates proanthocyanidin biosynthesis by repressing VrANR in mung bean (Vigna radiata L.).Planta · 2025Article
- SbC1, an R2R3-MYB transcription factor, specifically regulates anthocyanin accumulation in sorghum coleoptiles.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Article
- Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenousaBIOTECH · 2025Article
- Transcriptome analysis reveals biosynthesis and regulation of flavonoid in common bean seeds during grain filling.BMC plant biology · 2024Article
- PnMYB4 negatively modulates saponin biosynthesis inHorticulture research · 2023Article
- Transcriptomic-based analysis to identify candidate genes for blue color rose breeding.Plant molecular biology · 2023Article
- Enhancement of the anthocyanin contents ofFrontiers in plant science · 2023Article
- Eco-Evo-Devo of petal pigmentation patterning.Essays in biochemistry · 2022Article
- Transcription Factor CmNAC34 RegulatedInternational journal of molecular sciences · 2022Article
- MtGSTF7, a TT19-like GST gene, is essential for accumulation of anthocyanins, but not proanthocyanins in Medicago truncatula.Journal of experimental botany · 2022Article
- Integrated multi-omic data and analyses reveal the pathways underlying key ornamental traits in carnation flowers.Plant biotechnology journal · 2022Article
- Comparison of structural variants in the whole genome sequences of two Medicago truncatula ecotypes: Jemalong A17 and R108.BMC plant biology · 2022Article
- TcMYB29a, an ABA-Responsive R2R3-MYB Transcriptional Factor, Upregulates Taxol Biosynthesis inFrontiers in plant science · 2022Article
- The PeaFrontiers in genetics · 2022Article
- ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3'-hydroxylase gene inFrontiers in plant science · 2022Article
- Quantitative dissection of color patterning in the foliar ornamental coleus.Plant physiology · 2021Article
- Regulatory Mechanisms of bHLH Transcription Factors in Plant Adaptive Responses to Various Abiotic Stresses.Frontiers in plant science · 2021Review
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Patterned leaf coloration in plants generates remarkable diversity in nature, but the underlying mechanisms remain largely unclear. Here, using Medicago truncatula leaf marking as a model, we show that the classic M. truncatula leaf anthocyanin spot trait depends on two R2R3 MYB paralogous regulators, RED HEART1 (RH1) and RH2. RH1 mainly functions as an anthocyanin biosynthesis activator that specifically determines leaf marking formation depending on its C-terminal activation motif. RH1 physically interacts with the M. truncatula bHLH protein MtTT8 and the WDR family member MtWD40-1, and this interaction facilitates RH1 function in leaf anthocyanin marking formation. RH2 has lost transcriptional activation activity, due to a divergent C-terminal domain, but retains the ability to interact with the same partners, MtTT8 and MtWD40-1, as RH1, thereby acting as a competitor in the regulatory complex and exerting opposite effects. Moreover, our results demonstrate that RH1 can activate its own expression and that RH2-mediated competition can repress RH1 expression. Our findings reveal the molecular mechanism of the antagonistic gene paralogs RH1 and RH2 in determining anthocyanin leaf markings in M. truncatula, providing a multidimensional paralogous-antagonistic regulatory paradigm for fine-tuning patterned pigmentation.
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