Evidence map›Paper›PMID 33222131›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion.

David E Gordon, Amber S Shun-Shion, Asral W Asnawi, Andrew A Peden

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David E GordonDepartment of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA.
Amber S Shun-ShionDepartment of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield, UK.
Asral W AsnawiDepartment of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield, UK.
Andrew A PedenDepartment of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield, UK. a.peden@sheffield.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/J014443/1Biotechnology and Biological Sciences Research Council BB/L002841/1Biotechnology and Biological Sciences Research Council BB/L022389/1Biotechnology and Biological Sciences Research Council BB/S009566/1Medical Research Council G120/952
6 · The paper itself

Abstract

Constitutive secretion is predominantly measured by collecting the media from cells and performing plate-based assays. This approach is particularly sensitive to changes in cell number, and a significant amount of effort has to be spent to overcome this. We have developed a panel of quantitative flow cytometry-based assays and reporter cell lines that can be used to measure constitutive secretion. These assays are insensitive to changes in cell number making them very robust and well suited to functional genomic and chemical screens. Here, we outline the key steps involved in generating and using these assays for studying constitutive secretion.

Indexed as

Biological AssayBodily SecretionsCell LineFlow CytometryHumansAssayBiosynthetic transportConstitutive secretionFlow cytometryGPIhGHTraffickingTransportVSV-G

Identifiers

PMID33222131

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.