Evidence map›Paper›PMID 33222001›Full record

ArticleAnalytical and bioanalytical chemistry2021

Altered N-linked glycosylation in endometrial cancer.

Parul Mittal, Matthew Briggs, Manuela Klingler-Hoffmann, Gurjeet Kaur, Nicolle H Packer, Martin K Oehler, Peter Hoffmann

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Review
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  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Review
  12. Glycosylation and its research progress in endometrial cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2022
    Review
  13. Review
  14. Spatial Mapping of PlantFrontiers in plant science · 2022
    Article
  15. Article
  16. Article
  17. Article
  18. Review
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Parul MittalFuture Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA, 5095, Australia.
Matthew BriggsFuture Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA, 5095, Australia.
Manuela Klingler-HoffmannFuture Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA, 5095, Australia.
Gurjeet KaurInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Minden, Pulau Pinang, Malaysia.
Nicolle H PackerInstitute for Glycomics, Gold Coast Campus, Griffith University, Gold Coast, QLD, 4215, Australia.
Martin K OehlerDepartment of Gynaecological Oncology, Royal Adelaide Hospital, North Terrace, Adelaide, SA, 5000, Australia.
Peter HoffmannFuture Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA, 5095, Australia. peter.hoffmann@unisa.edu.au.
University of South Australia · AUGriffith University · AURoyal Adelaide Hospital · AUUniversiti Sains Malaysia · MY

Funding

Bioplatforms Australia n/a
6 · The paper itself

Abstract

It is well established that cell surface glycans play a vital role in biological processes and their altered form can lead to carcinogenesis. Mass spectrometry-based techniques have become prominent for analysing N-linked glycans, for example using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Additionally, MALDI MS can be used to spatially map N-linked glycans directly from cancer tissue using a technique termed MALDI MS imaging (MALDI MSI). This powerful technique combines mass spectrometry and histology to visualise the spatial distribution of N-linked glycans on a single tissue section. Here, we performed N-glycan MALDI MSI on six endometrial cancer (EC) formalin-fixed paraffin-embedded (FFPE) tissue sections and tissue microarrays (TMA) consisting of eight EC patients with lymph node metastasis (LNM) and twenty without LNM. By doing so, several putative N-linked glycan compositions were detected that could significantly distinguish normal from cancerous endometrium. Furthermore, a complex core-fucosylated N-linked glycan was detected that could discriminate a primary tumour with and without LNM. Structural identification of these putative N-linked glycans was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS). Overall, we observed higher abundance of oligomannose glycans in tumour compared to normal regions with AUC ranging from 0.85-0.99, and lower abundance of complex N-linked glycans with AUC ranges from 0.03-0.28. A comparison of N-linked glycans between primary tumours with and without LNM indicated a reduced abundance of a complex core-fucosylated N-glycan (Hex)

Indexed as

Endometrial NeoplasmsEndometriumFemaleFormaldehydeGlycosylationHumansPolysaccharidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTissue Array AnalysisTissue FixationFormaldehydePolysaccharidesBiomarkerEndometrial cancerGlycan imagingLymph node metastasis

Identifiers

PMID33222001
OpenAlexW3107382989

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.