Evidence map›Paper›PMID 33220500›Full record

ArticleMitochondrion2021

Measurement of mitochondrial DNA copy number in dried blood spots: A pilot study.

Chelsea Anderson, Rebecca C Fry, Hadley Hartwell, Cynthia Kleeberger, Dale P Sandler, Hazel B Nichols

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Mitochondrion, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Extreme prematurity: Risk and resiliency.Current problems in pediatric and adolescent health care · 2022
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Chelsea AndersonDepartment of Epidemiology, University of North Carolina, Chapel Hill, NC, USA.
Rebecca C FryDepartment of Environmental Science and Engineering, University of North Carolina, Chapel Hill, NC, USA.
Hadley HartwellDepartment of Environmental Science and Engineering, University of North Carolina, Chapel Hill, NC, USA.
Cynthia KleebergerSocial & Scientific Systems, Durham, NC, USA.
Dale P SandlerNational Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.
Hazel B NicholsDepartment of Epidemiology, University of North Carolina, Chapel Hill, NC, USA. Electronic address: hazel.nichols@unc.edu.
University of North Carolina at Chapel Hill · USSocial and Scientific Systems (United States) · USTriangle · US

Funding

UNC-CH CENTER FOR ENVIRONMENTAL HEALTH &SUSCEPTIBILITYP30ES010126 · NIEHS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Hazel B Nichols · 2001 to 2026
$36.3M
Environmental and Genetic Risk Factors For Breast Cancer: The Sister StudyZIAES044005 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI SANDLER, DALE P · 2009 to 2025
$22.7M
Cancer Control Education ProgramT32CA057726 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Melissa B Gilkey, Melissa A. Troester · 2017 to 2026
$3.7M
Genetic and Environmental Risk Factors for Breast Cancer: The Sister StudyZ01ES044005 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI SANDLER, DALE P · 1999 to 2008
$1.3M
Intramural NIH HHS Z01 ES044005NCI NIH HHS T32 CA057726NIEHS NIH HHS P30 ES010126
6 · The paper itself

Abstract

backgroundWe evaluated the feasibility of mitochondrial DNA (mtDNA) copy number measurement in dried blood spots (DBS), its comparability with measurement in whole blood samples, and stability of mtDNA copy number from DBS over time.

methodsWomen in this pilot study were participants in the Sister Study, a large prospective cohort. Sister Study participants provided a whole blood sample and DBS at enrollment. A second DBS sample was collected 5-10 years later from a subcohort of women with and without an incident breast cancer diagnosis between collections. Among 54 women (27 with breast cancer, 27 without) we measured mtDNA copy number from whole blood at enrollment and from DBS at both time points.

resultsThe average age at enrollment was 58.7 years (range:50-69). Values of mtDNA copy number measured in whole blood samples and DBS from enrollment were moderately correlated (Spearman R = 0.45; p = 0.005). Stability of mtDNA copy number in DBS from the two time points was moderate overall (ICC = 0.50) and similar between women with (ICC = 0.50) and without (ICC = 0.51) a breast cancer diagnosis between the two collections.

conclusionsOur results suggest that measurement of mtDNA copy number in DBS is feasible and may be a valid alternative to measurement in whole blood samples.

Indexed as

DNA Copy Number VariationsAgedBlood Specimen CollectionBreast NeoplasmsCase-Control StudiesDNA, MitochondrialFeasibility StudiesFemaleHumansMiddle AgedMitochondriaPilot ProjectsProspective StudiesDNA, MitochondrialDried blood spotsHumansMitochondrial DNA copy number

Identifiers

PMID33220500
PMCPMC8381758
OpenAlexW3109619467

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.