ArticleEuropean biophysics journal : EBJ2020
Multi-wavelength analytical ultracentrifugation as a tool to characterise protein-DNA interactions in solution.
Article in European biophysics journal : EBJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Mechanism of NanR transcriptional activation of sialic acid metabolism in Streptococcus pneumoniae.Nucleic acids research · 2026Article
- Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments.Current protocols · 2024Article
- Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan.Nanomedicine (London, England) · 2023Article
- Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs.European biophysics journal : EBJ · 2023Article
- Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation.European biophysics journal : EBJ · 2023Article
- Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria.Nature communications · 2022Article
- Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions.Analytical biochemistry · 2022Review
- Using cryo-EM to uncover mechanisms of bacterial transcriptional regulation.Biochemical Society transactions · 2021Review
- N-acetylmannosamine-6-phosphate 2-epimerase uses a novel substrate-assisted mechanism to catalyze amino sugar epimerization.The Journal of biological chemistry · 2021Article
- Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism.Nature communications · 2021Article
- Analytical ultracentrifugation: still the gold standard that offers multiple solutions.European biophysics journal : EBJ · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 4 countries.
Funding
Abstract
Understanding how proteins interact with DNA, and particularly the stoichiometry of a protein-DNA complex, is key information needed to elucidate the biological role of the interaction, e.g. transcriptional regulation. Here, we present an emerging analytical ultracentrifugation method that features multi-wavelength detection to characterise complex mixtures by deconvoluting the spectral signals of the interaction partners into separate sedimentation profiles. The spectral information obtained in this experiment provides direct access to the molar stoichiometry of the interacting system to complement traditional hydrodynamic information. We demonstrate this approach by characterising a multimeric assembly process between the transcriptional repressor of bacterial sialic acid metabolism, NanR and its DNA-binding sequence. The method introduced in this study can be extended to quantitatively analyse any complex interaction in solution, providing the interaction partners have different optical properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.