ArticleNature chemistry2020
Enzyme-mediated nitric oxide production in vasoactive erythrocyte membrane-enclosed coacervate protocells.
Article in Nature chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 218 citations in OpenAlex.
- Biomolecular coacervation-mediated materials: Phase states, phase transitions, and biomedical applications.Bioactive materials · 2027Review
- Functionalized Coacervates for Cancer Therapy: Advances and Prospects in Phase-Separation-Based Intelligent Drug Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Increasing the Compositional Heterogeneity of Single-Chain Amphiphile Membranes Supported by Coacervate Cores Alters Stability and Properties of the Hybrid Protocells.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Dynamic Covalent Boronate Chemistry forJournal of the American Chemical Society · 2026Article
- Recent advances in coacervate protocells from passive catalysts to chemically programmable systems.Communications chemistry · 2026Review
- Pulmonary-Targeted Nanoparticles Interrupt the Malignant Mechanical and Biochemical Signaling Crosstalk for Idiopathic Pulmonary Fibrosis Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Design and applications of synthetic biomolecular condensates.Nature nanotechnology · 2026Review
- A microgel-stabilized, light-controlled artificial energy supply module for efficient biosynthesis.Regenerative biomaterials · 2026Article
- Self-Coacervation of Oligo(ethylene glycol) and Oligo(2-ethyl-2-oxazoline)-Based Double Hydrophilic Brush Block Copolymers in Aqueous Solution.Macromolecular rapid communications · 2025Article
- Phase-Separated Coacervates of Low-Molecular-Weight Compounds for Cytosolic Delivery and Disease Treatment.JACS Au · 2025Review
- Automated navigation of condensate phase behavior with active machine learning.Nature communications · 2025Article
- Photoactivatable Synthetic Exosomes for RNA-Based Communication Between Artificial Cells and Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Biomolecular Condensates as Emerging Biomaterials: Functional Mechanisms and Advances in Computational and Experimental Approaches.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Dendritic Membranized Coacervate Microdroplets: A Robust Platform for Synthetic-Living Cell Consortia.Journal of the American Chemical Society · 2025Article
- A closed-loop bioelectronic patch for intelligent blood pressure management.Science advances · 2025Article
- Coacervate-Based Delivery Systems: Bridging Fundamentals and Applications.ACS applied materials & interfaces · 2025Review
- Interfacial assembly of biomimetic MOF-based porous membranes on coacervates to build complex protocells and prototissues.Nature chemistry · 2025Article
- Artificial multienzyme nanoflower composite hydrogel for efficiently promoting MRSA-infected diabetic wound healing via glucose-activated NO releasing and microenvironment regulation.Bioactive materials · 2025Article
- Universal membranization of synthetic coacervates and biomolecular condensates towards ultrastability and spontaneous emulsification.Nature chemistry · 2025Article
- Cytomimetic calcification in chemically self-regulated prototissues.Nature communications · 2025Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
The design and construction of synthetic therapeutic protocells capable of establishing cognate chemical communication channels with living cells is an important challenge for synthetic biology and bio-engineering. Here we develop a step towards protocell-mediated nitric-oxide-induced vasodilation by constructing a new synthetic cell model based on bio-derived coacervate vesicles with high haemocompatibility and increased blood circulation times. The hybrid protocells are prepared by the spontaneous self-assembly of haemoglobin-containing erythrocyte membrane fragments on the surface of preformed polysaccharide-polynucleotide coacervate micro-droplets containing glucose oxidase. We use the sequestered enzymes to program a spatially coupled glucose oxidase/haemoglobin reaction cascade, which in the presence of glucose and hydroxyurea generates a protocell-mediated flux of nitric oxide that we exploit for in vitro and in vivo blood vessel vasodilation. Taken together, our results provide new opportunities for the development of endogenously organized cell-like entities (biocompatible micro-bots) geared specifically towards active interfacing with individual living cells and cell communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.