ArticleScientific reports2020
Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 44 citations in OpenAlex.
- Rhizome differentiation is associated with metabolic specialization and rhizosphere microbial assembly in Rheum officinale Baill.Plant cell reports · 2026Article
- Comparative genomics reveals the evolution of anthraquinone biosynthesis in Polygonaceae.BMC genomics · 2026Article
- Active ingredients, nutrition values and health-promoting effects of aboveground parts of rhubarb: a review.Food science and biotechnology · 2026Review
- Dissecting alternative splicing patterns of the Rheum palmatum complex with different contents of medicinal constituents.BMC genomics · 2025Article
- Mechanistic Elucidation and Establishment of Drying Kinetic Models of Differential Metabolite Regulation inBiology · 2025Article
- Green Extraction Strategies and Bioactivity ofPlants (Basel, Switzerland) · 2025Article
- Recent advances in Polygonaceae endophytes: diversity, secondary metabolites and biotechnological applications.Frontiers in bioengineering and biotechnology · 2025Review
- Integrated metabolite profiling and transcriptome analysis identify candidate genes involved in diterpenoid alkaloid biosynthesis inFrontiers in plant science · 2025Article
- Identification of Secondary Metabolites by UHPLC-ESI-HRMS/MS in Antifungal StrainJournal of fungi (Basel, Switzerland) · 2024Article
- Comparative transcriptome analysis and HPLC reveal candidate genes associated with synthesis of bioactive constituents inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Enzymatic glycosylation of aloesone performed by plant UDP-dependent glycosyltransferases.Glycobiology · 2024Article
- A chromosome-level genome reveals genome evolution and molecular basis of anthraquinone biosynthesis in Rheum palmatum.BMC plant biology · 2024Article
- Transcriptomic divergence of the Rheum palmatum complex derived from top-geoherb and non-geoherb areas provides the insights into geoherbalism properties of rhubarb.BMC genomics · 2024Article
- The haplotype-resolved genome assembly of autotetraploid rhubarb Rheum officinale provides insights into its genome evolution and massive accumulation of anthraquinones.Plant communications · 2024Article
- Molecular Traits Underlying the Growth Promotion and Metabolite Accumulation inInternational journal of molecular sciences · 2022Article
- Comparative Transcriptome Analyses of DifferentGenes · 2022Article
- Comparative Metabolomics study of flavonoids in the pericarp of different coloured bitter gourds (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis.BMC plant biology · 2022Article
- Integrated transcriptome and metabolome analyses revealed regulatory mechanisms of flavonoid biosynthesis inPeerJ · 2022Article
- Comparative transcriptome analysis of different tissues of Rheum tanguticum Maxim. ex Balf. (Polygonaceae) reveals putative genes involved in anthraquinone biosynthesis.Genetics and molecular biology · 2022Article
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7 authors at 1 institution in 1 country.
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Abstract
Rheum emodi is a perennial herb and an important medicinal plant, with anthraquinones and flavonoids as its main bioactive compounds. However, there is little knowledge about the biosynthetic pathway of anthraquinones in rhubarbs. In this study, we qualitatively and quantitatively assessed 62 pharmacological metabolites in rhubarb using dynamic multiple reaction monitoring (dMRM) of triple-quadrupole mass spectrometry (QqQ-MS), including 21 anthraquinones, 17 flavonoids, 6 stilbenes, 12 gallate esters, 3 tannins, and 3 others. Besides, the metabolomics results showed significant differences among all the 60 metabolites, except for gallic acid and piceatannol-O-β-glucoside. The combined transcriptome data of R. palmatum L. (RPL) and R. officinale Baill. (ROB) showed that 21,691 unigenes were annotated in the metabolic pathways. Taken together, 17 differentially expressed genes (DEGs) were associated with the anthraquinone biosynthetic pathway. Additionally, a significant correlation between anthraquinone peak intensity and DEG expression level existed, validating that DEGs contribute to the anthraquinone biosynthetic pathway. RT-qPCR results showed that the cluster-14354.38156 gene may catalyze the O-methylation of emodin to produce physcion. This study provides a useful resource for further studies on secondary metabolism in rhubarb and the combination analysis of transcriptome and metabolome, which can help with the discovery of enzyme genes involved in metabolite biosynthesis.
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