ArticleThe New phytologist2021
F-box protein CFK1 interacts with and degrades de novo DNA methyltransferase in Arabidopsis.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Time-dependent regulation of ABA and JA in starch accumulation during the natural development of GZ 1 grains.Molecular biology reports · 2025Article
- Substrate specificity and protein stability drive the divergence of plant-specific DNA methyltransferases.Science advances · 2024Article
- Arabidopsis F-box proteins D5BF1 and D5BF2 negatively regulate Agrobacterium-mediated transformation and tumorigenesis.Molecular plant pathology · 2024Article
- Genetic and environmental drivers of large-scale epigenetic variation in Thlaspi arvense.PLoS genetics · 2022Article
- Genetic analysis of the early bud flush trait of tea plants (Horticulture research · 2022Article
- Structure and Mechanism of Plant DNA Methyltransferases.Advances in experimental medicine and biology · 2022Article
- Diverse Evolution in 111 Plant Genomes Reveals Purifying and Dosage Balancing Selection Models forInternational journal of molecular sciences · 2021Article
- Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.Frontiers in plant science · 2021Review
- A Machine Learning Approach to Prioritizing Functionally ActiveFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
DNA methylation plays crucial roles in cellular development and stress responses through gene regulation and genome stability control. Precise regulation of DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2), the de novo Arabidopsis DNA methyltransferase, is crucial to maintain DNA methylation homeostasis to ensure genome integrity. Compared with the extensive studies on DRM2 targeting mechanisms, little information is known regarding the quality control of DRM2 itself. Here, we conducted yeast two-hybrid screen assay and identified an E3 ligase, COP9 INTERACTING F-BOX KELCH 1 (CFK1), as a novel DRM2-interacting partner and targets DRM2 for degradation via the ubiquitin-26S proteasome pathway in Arabidopsis thaliana. We also performed whole genome bisulfite sequencing (BS-seq) to determine the biological significance of CFK1-mediated DRM2 degradation. Loss-of-function CFK1 leads to increased DRM2 protein abundance and overexpression of CFK1 showed reduced DRM2 protein levels. Consistently, CFK1 overexpression induces genome-wide CHH hypomethylation and transcriptional de-repression at specific DRM2 target loci. This study uncovered a distinct mechanism regulating de novo DNA methyltransferase by CFK1 to control DNA methylation level.
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Registered trials
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