ArticleJournal of experimental botany2021
Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.
Article in Journal of experimental botany, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Multi-Scale Remote-Sensing Phenomics Integrated with Multi-Omics: Advances in Crop Drought-Heat Stress Tolerance Mechanisms and Perspectives for Climate-Smart Agriculture.Plants (Basel, Switzerland) · 2025Review
- Alternaria solani Effector AsCEP20, Essential for Virulence, Targets Potato StFtsH4 Protein to Suppress Plant Disease Resistance.Molecular plant pathology · 2025Article
- Genome-wide analysis of filamentous temperature-sensitive H protease (ftsH) gene family in soybean.BMC genomics · 2024Article
- Degradation of FATTY ACID EXPORT PROTEIN1 by RHOMBOID-LIKE PROTEASE11 contributes to cold tolerance in Arabidopsis.The Plant cell · 2024Article
- Natural genetic variation in GLK1-mediated photosynthetic acclimation in response to light.BMC plant biology · 2024Article
- Chloroplast import motor subunits FtsHi1 and FtsHi2 are located on opposite sides of the inner envelope membrane.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- An evolutionary look into the history of lentil reveals unexpected diversity.Evolutionary applications · 2022Article
- The Eucalyptus grandis chloroplast proteome: Seasonal variations in leaf development.PloS one · 2022Article
- The essential roles of OsFtsH2 in developing the chloroplast of rice.BMC plant biology · 2021Article
- The FtsHi Enzymes ofInternational journal of molecular sciences · 2021Review
- Plant proteases: from molecular mechanisms to functions in development and immunity.Journal of experimental botany · 2021Article
- MANTI: Automated Annotation of Protein N-Termini for Rapid Interpretation of N-Terminome Data Sets.Analytical chemistry · 2021Article
- The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis.Frontiers in plant science · 2021Article
- To New Beginnings: Riboproteogenomics Discovery of N-Terminal Proteoforms inFrontiers in plant science · 2021Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ATP-dependent metalloprotease FtsH12 (filamentation temperature sensitive protein H 12) has been suggested to participate in a heteromeric motor complex, driving protein translocation into the chloroplast. FtsH12 was immuno-detected in proplastids, seedlings, leaves, and roots. Expression of Myc-tagged FtsH12 under its native promotor allowed identification of FtsHi1, 2, 4, and 5, and plastidic NAD-malate dehydrogenase, five of the six interaction partners in the suggested import motor complex. Arabidopsis thaliana mutant seedlings with reduced FTSH12 abundance exhibited pale cotyledons and small, deformed chloroplasts with altered thylakoid structure. Mature plants retained these chloroplast defects, resulting in slightly variegated leaves and lower chlorophyll content. Label-free proteomics revealed strong changes in the proteome composition of FTSH12 knock-down seedlings, reflecting impaired plastid development. The composition of the translocon on the inner chloroplast membrane (TIC) protein import complex was altered, with coordinated reduction of the FtsH12-FtsHi complex subunits and accumulation of the 1 MDa TIC complex subunits TIC56, TIC214 and TIC22-III. FTSH12 overexpressor lines showed no obvious phenotype, but still displayed distinct differences in their proteome. N-terminome analyses further demonstrated normal proteolytic maturation of plastid-imported proteins irrespective of FTSH12 abundance. Together, our data suggest that FtsH12 has highest impact during seedling development; its abundance alters the plastid import machinery and impairs chloroplast development.
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