ArticleJournal of mammary gland biology and neoplasia2020
Single Cell RNA Sequencing of Human Milk-Derived Cells Reveals Sub-Populations of Mammary Epithelial Cells with Molecular Signatures of Progenitor and Mature States: a Novel, Non-invasive Framework for Investigating Human Lactation Physiology.
Article in Journal of mammary gland biology and neoplasia, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The trial behind it
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Who cites it
48 citing papers in PubMed, 57 citations in OpenAlex.
- Trial
- Dysregulated Efferocytosis and Immune Microenvironment in High Somatic Cell Count Dairy Cows.Journal of animal physiology and animal nutrition · 2026Article
- SOX9 and SEMA7A regulate cell plasticity in the postpartum mammary gland with implications for breast cancer.bioRxiv : the preprint server for biology · 2026Article
- Article
- Methods for Preserving Human Milk Cellular and Milk Fat Globule RNA.Journal of mammary gland biology and neoplasia · 2026Article
- Functional shifts in immune composition follow lactation stage in human milk.Frontiers in immunology · 2026Article
- Impact of lactation stage, maternal age, and parity on mammary epithelial cell populations in fresh milk.Scientific reports · 2025Article
- A single-cell transcriptomic study of milk cells from dairy cows with divergent lactation performance.Scientific reports · 2025Article
- Integrated 'omics analysis reveals human milk oligosaccharide biosynthesis programs in human lactocytes.iScience · 2025Article
- Genomic characterization of normal and aberrant human milk production.Science advances · 2025Article
- Defining breast epithelial cell types in the single-cell era.Developmental cell · 2025Review
- Human milk extracellular vesicles modulate inflammation and cell survival in intestinal and immune cells.Pediatric research · 2025Article
- Volumetric printed biomimetic scaffolds support in vitro lactation of human milk-derived mammary epithelial cells.Science advances · 2025Article
- Comparison Between SGLT2 Inhibitors and Lactation: Implications for Cardiometabolic Health in Parous Women.Metabolic syndrome and related disorders · 2025Review
- Challenges and Opportunities in Quantifying Bioactive Compounds in Human Breastmilk.Biomolecules · 2025Review
- Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing.Clinical and experimental pediatrics · 2025Article
- Maternal milk cell components are uptaken by infant liver macrophages via extracellular vesicle mediated transport.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications.World journal of microbiology & biotechnology · 2025Review
- Human milk variation is shaped by maternal genetics and impacts the infant gut microbiome.Cell genomics · 2024Article
- Human cytomegalovirus in breast milk is associated with milk composition and the infant gut microbiome and growth.Nature communications · 2024Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Cells in human milk are an untapped source, as potential "liquid breast biopsies", of material for investigating lactation physiology in a non-invasive manner. We used single cell RNA sequencing (scRNA-seq) to identify milk-derived mammary epithelial cells (MECs) and their transcriptional signatures in women with diet-controlled gestational diabetes (GDM) with normal lactation. Methodology is described for coordinating milk collections with single cell capture and library preparation via cryopreservation, in addition to scRNA-seq data processing and analyses of MEC transcriptional signatures. We comprehensively characterized 3740 cells from milk samples from two mothers at two weeks postpartum. Most cells (>90%) were luminal MECs (luMECs) expressing lactalbumin alpha and casein beta and positive for keratin 8 and keratin 18. Few cells were keratin 14
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.