ArticlePloS one2020
Overexpression of MdCPK1a gene, a calcium dependent protein kinase in apple, increase tobacco cold tolerance via scavenging ROS accumulation.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 65 citations in OpenAlex.
- Phosphorylation of VpCAMTA3 by VpCDPK13 enhances cold tolerance in grapevine.Horticulture research · 2026Article
- Regulatory insights underlying apple to cold stress.Horticulture research · 2026Article
- Calcium-Dependent Protein Kinases in Plants: Structure, Signaling, and Multifaceted Regulatory Roles in Development and Stress Adaptation.International journal of molecular sciences · 2026Review
- Cold-tolerant bacteria from alpine Rosaceae plants modulate transcriptional responses of apple and strawberry plantlets to freezing stress.Frontiers in plant science · 2026Article
- Functional Analysis ofCurrent issues in molecular biology · 2025Article
- Differential Modulation of Brassinosteroid and Ethylene Signalling Systems by Native and Constitutively Active Forms of thePlants (Basel, Switzerland) · 2025Article
- Calcium-dependent protein kinases as central hubs in plant abiotic stress signaling: mechanisms and prospects for crop improvement.Frontiers in plant science · 2025Review
- Article
- Overexpression ofInternational journal of molecular sciences · 2024Article
- Effects of copper, and aluminium in ionic, and nanoparticulate form on growth rate and gene expression of Setaria italica seedlings.Scientific reports · 2024Article
- Functional Identification ofPlants (Basel, Switzerland) · 2024Article
- Article
- Identification ofFrontiers in plant science · 2024Article
- Genome-wide analysis of the CDPK gene family and their important roles response to cold stress in white clover.Plant signaling & behavior · 2023Article
- The positive impact of the NtTAS14-like1 gene on osmotic stress response in Nicotiana tabacum.Plant cell reports · 2023Article
- Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit.International journal of molecular sciences · 2023Article
- Multi-Omics Revealed Peanut Root Metabolism Regulated by Exogenous Calcium under Salt Stress.Plants (Basel, Switzerland) · 2023Article
- Whole-Genome Identification of Regulatory Function of CDPK Gene Families in Cold Stress Response forPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Analysis ofPlants (Basel, Switzerland) · 2023Article
- Abiotic factors and endophytes co-regulate flavone and terpenoid glycoside metabolism in Glycyrrhiza uralensis.Applied microbiology and biotechnology · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium-dependent protein kinases (CDPKs) are important calcium receptors, which play a crucial part in the process of sensing and decoding intracellular calcium signals during plant development and adaptation to various environmental stresses. In this study, a CDPK gene MdCPK1a, was isolated from apple (Malus×domestica) which contains 1701bp nucleotide and encodes a protein of 566 amino acid residues, and contains the conserved domain of CDPKs. The transient expression and western blot experiment showed that MdCPK1a protein was localized in the nucleus and cell plasma membrane. Ectopic expression of MdCPK1a in Nicotiana benthamiana increased the resistance of the tobacco plants to salt and cold stresses. The mechanism of MdCPK1a regulating cold resistance was further investigated. The overexpressed MdCPK1a tobacco plants had higher survival rates and longer root length than wild type (WT) plants under cold stress, and the electrolyte leakages (EL), the content of malondialdehyde (MDA) and reactive oxygen species (ROS) were lower, and accordingly, antioxidant enzyme activities, such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were higher, suggesting the transgenic plants suffered less chilling injury than WT plants. Moreover, the transcript levels of ROS-scavenging and stress-related genes were higher in the transgenic plants than those in WT plants whether under normal conditions or cold stress. The above results suggest that the improvement of cold tolerance in MdCPK1a-overexpressed plants was due to scavenging ROS accumulation and modulating the expression of stress-related genes.
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