Evidence map›Paper›PMID 33210246›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines.

Karim Rahimi, Sara Parsa, Mehrnoush Nikzaban, Behnoush Khaledian, Seyed Javad Mowla, Fardin Fathi

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Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Karim RahimiDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Sara ParsaMolecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Mehrnoush NikzabanDepartment of Biological Sciences and Biotechnology, Faculty of Science, University of Kurdistan, Sanandaj, Iran.
Behnoush KhaledianCellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Seyed Javad MowlaMolecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Fardin FathiCellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. farfath@gmail.com.ORCID http://orcid.org/0000-0002-4648-5598
Kurdistan University of Medical Sciences · IRTarbiat Modares University · IRAarhus University · DKUniversity of Kurdistan · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Some miRNAs, including the miR-302 cluster, are critical regulators of the stemness state of embryonic stem cells and cell fate patterning. In this study, we evaluated the activity of the miR-302 core promotor in mice and human pluripotent stem cells, somatic tissue derivatives, and generated transgenic mice expressing EGFP under a miR-302 promoter. The expression of EGFP under the control of the miR-302 promotor was examined in the cell lines and somatic tissues of transgenic mice, transgenic blastocysts, and embryonic stem cells derived from transgenic blastocysts. Our results showed that the miR-302 promoter is highly expressed in the mouse and human pluripotent cells, weakly expressed in the somatic tissue derivatives, is highly expressed in both blastocysts and the first passages of transgenic embryonic stem cells, and lowly expressed in the somatic tissues of transgenic mice. It can be concluded that different temporal and spatial gene expression patterns occur during the embryonic and adult stages of cells in mice.

Indexed as

Promoter Regions, GeneticAnimalsBlastocystCell LineGene Expression RegulationGenotypeGreen Fluorescent ProteinsHumansMiceMice, TransgenicMicroRNAsMouse Embryonic Stem CellsPluripotent Stem Cellsenhanced green fluorescent proteinGreen Fluorescent ProteinsMicroRNAsMIRN302A microRNA, humanAdult stem cellsBlastocystEmbryonic stem cellsMicroRNAsTransgenic mice

Identifiers

PMID33210246
OpenAlexW3099770627

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.