Evidence map›Paper›PMID 33209874›Full record

ArticleAnnals of translational medicine2020

LncRNA HULC shRNA disinhibits miR-377-5p to suppress the growth and invasion of hepatocellular carcinoma

Chunxiao Yan, Shutang Wei, Dazheng Han, Liping Wu, Lixia Tan, Hangyu Wang, Yong Dong, Jing Hua, Wenyi Yang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of translational medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Roles of long non-coding RNAFrontiers in oncology · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. The lncRNAJournal of inflammation research · 2021
    Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Chunxiao YanDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Shutang WeiDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Dazheng HanDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Liping WuDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Lixia TanDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Hangyu WangDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Yong DongDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Jing HuaDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
Wenyi YangDepartment of Gastroenterology, The First Affiliated Hospital of Henan University, Kaifeng, China.
First Affiliated Hospital of Henan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAberrant expression of up-regulated long non-coding RNA [LncRNA highly upregulated in liver cancer (HULC)] has been observed to play an important regulatory role in the development of multiple human diseases. However, the molecular mechanism underlying the role of HULC and miR-377-5p in HCC needs to be urgently explored.

methodsThe mRNA and protein expression levels of HULC were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot in hepatocellular carcinoma (HCC) cell line HB611, HepG2 and H22, respectively. HULC-shRNA was transfected into HepG-2 cells, which were randomly divided into the control, shRNA-NC, and sh-HULC groups. The correlation between HULC and miR-377-5p was analyzed by performing a luciferase reporter assay. The targeting relationship between miR-377-5p and hypoxia-inhibitory factor-1α (HIF-1α) was also investigated using a luciferase reporter assay. Sh-HULC and miR-377-5p inhibitors were transfected either alone or together into HepG2 cells, and which were divided into the control group, the sh-HULC group, the miR-377-5p inhibitor, and the sh-HULC + inhibitor group for subsequent experiments. HepG2 cell proliferation and invasion were measured by 5-Ethynyl-2-Deoxyuridine (EdU) staining and Transwell invasion assay, respectively. Western plot was carried out to detect the protein expression levels of Ki67, PCNA, E-cadherin, and N-cadherin. Tumor xenograft mouse models were established to confirm the effect of HULC down-regulation on the development of HCC

resultsThe mRNA and protein expression levels of HULC were markedly increased, whereas the mRNA expression levels of miR-377-5p were decreased in HCC cell lines. HepG2 cell proliferation and invasion were suppressed in the Sh-HULC group, while miR-377-5p showed the opposite. Further experiments exhibited that miR-377-5p was targeted by HULC, and an negative correlation between HULC and miR-377-5p was observed. Importantly, the

conclusionsIn summary, our

Indexed as

hepatocellular carcinoma (HCC)Highly upregulated in liver cancer (HULC)invasion, apoptosis, hepatocarcinogenesismiR-377-5p

Identifiers

PMID33209874
PMCPMC7661872
OpenAlexW3094764519

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.