Evidence map›Paper›PMID 33209324›Full record

ArticleIUCrJ2020

Low-dose

Isabelle Martiel, Chia-Ying Huang, Pablo Villanueva-Perez, Ezequiel Panepucci, Shibom Basu, Martin Caffrey, Bill Pedrini, Oliver Bunk, Marco Stampanoni, Meitian Wang

Abstract read
In one paragraph

Article in IUCrJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Isabelle MartielPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.ORCID https://orcid.org/0000-0003-1318-3830
Chia-Ying HuangPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.ORCID https://orcid.org/0000-0002-7676-0239
Pablo Villanueva-PerezPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.
Ezequiel PanepucciPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.ORCID https://orcid.org/0000-0003-3388-997X
Shibom BasuPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.
Martin CaffreySchool of Medicine and School of Biochemistry and Immunology, Trinity College, Dublin 2, D02 R590, Ireland.ORCID https://orcid.org/0000-0002-2931-4551
Bill PedriniPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.
Oliver BunkPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.ORCID https://orcid.org/0000-0001-6563-4053
Marco StampanoniPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.
Meitian WangPaul Scherrer Institute, Forschungsstrasse 111, Villigen, 5232, Switzerland.ORCID https://orcid.org/0000-0002-5340-3036

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Serial protein crystallography has emerged as a powerful method of data collection on small crystals from challenging targets, such as membrane proteins. Multiple microcrystals need to be located on large and often flat mounts while exposing them to an X-ray dose that is as low as possible. A crystal-prelocation method is demonstrated here using low-dose 2D full-field propagation-based X-ray phase-contrast imaging at the X-ray imaging beamline TOMCAT at the Swiss Light Source (SLS). This imaging step provides microcrystal coordinates for automated serial data collection at a microfocus macromolecular crystallography beamline on samples with an essentially flat geometry. This prelocation method was applied to microcrystals of a soluble protein and a membrane protein, grown in a commonly used double-sandwich

Indexed as

automated data collectionflat geometrymacromolecular crystallography beamlinesmembrane proteinsprelocationserial crystallographystructural biologyX-ray imaging

Identifiers

PMID33209324
PMCPMC7642777

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.