ArticleChemical science2020
Imaging effects of hyperosmolality on individual tricellular junctions.
Article in Chemical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Association between serum osmolality trajectories and mortality in sepsis patients: a retrospective multi-cohort study.European journal of medical research · 2025Article
- Trajectory of serum osmolality changes and 28-day mortality risk in patients with sepsis-associated delirium based on the MIMIC-IV database.Scientific reports · 2025Article
- Trajectories of blood urea nitrogen and 28-day mortality in patients with sepsis-associated delirium: a retrospective cohort analysis of the MIMIC-IV database.BMC infectious diseases · 2025Article
- The U-shaped relationship between serum osmolality and the risk of sepsis-associated delirium development: a retrospective study.BMC neurology · 2025Article
- Single-Cell Analysis with Spatiotemporal Control of Local pH.ACS measurement science au · 2025Article
- Analysis of the correlation between the group-based trajectory modeling of serum osmolality and prognosis in patients with sepsis-associated encephalopathy at 72 h after admission.BMC infectious diseases · 2025Article
- Association Between Blood Urea Nitrogen and Delirium in Critically Ill Elderly Patients Without Kidney Diseases: A Retrospective Study and Mendelian Randomization Analysis.CNS neuroscience & therapeutics · 2025Article
- The U-shaped association between serum osmolality and 28-day mortality in patients with sepsis: a retrospective cohort study.Infection · 2024Article
- Precision Delivery Using Nanopipette for Single-cell Studies.Dian hua xue · 2024Article
- 3D printed devices with integrated collagen scaffolds for cell culture studies including transepithelial/transendothelial electrical resistance (TEER) measurements.Analytica chimica acta · 2022Article
- Acute Effects of Focused Ultrasound-Induced Blood-Brain Barrier Opening on Anti-Pyroglu3 Abeta Antibody Delivery and Immune Responses.Biomolecules · 2022Article
- Changes in transepithelial electrical resistance and intracellular ion concentration in TGF-β-induced epithelial-mesenchymal transition of retinal pigment epithelial cells.American journal of translational research · 2022Article
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- A Hybrid Nanofiber/Paper Cell Culture Platform for Building a 3D Blood-brain Barrier Model.Small methods · 2021Article
- Potentially Detrimental Effects of Hyperosmolality in Patients Treated for Traumatic Brain Injury.Journal of clinical medicine · 2021Review
- Sauna dehydration as a new physiological challenge model for intestinal barrier function.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The use of hyperosmolar agents (osmotherapy) has been a major treatment for intracranial hypertension, which occurs frequently in brain diseases or trauma. However, side-effects of osmotherapy on the brain, especially on the blood-brain barrier (BBB) are still not fully understood. Hyperosmolar conditions, termed hyperosmolality here, are known to transiently disrupt the tight junctions (TJs) at the endothelium of the BBB resulting in loss of BBB function. Present techniques for evaluation of BBB transport typically reveal aggregated responses from the entirety of BBB transport components, with little or no opportunity to evaluate heterogeneity present in the system. In this study, we utilized potentiometric-scanning ion conductance microscopy (P-SICM) to acquire nanometer-scale conductance maps of Madin-Darby Canine Kidney strain II (MDCKII) cells under hyperosmolality, from which two types of TJs, bicellular tight junctions (bTJs) and tricellular tight junctions (tTJs), can be visualized and differentiated. We discovered that hyperosmolality leads to increased conductance at tTJs without significant alteration in conductance at bTJs. To quantify this effect, an automated computer vision algorithm was designed to extract and calculate conductance components at both tTJs and bTJs. Additionally, lowering Ca
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.