ArticleJournal of oral microbiology2020
Isolation and cultivation of candidate phyla radiation
Article in Journal of oral microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Modulator therapy: rates and stages of respiratory bacteriome restoration in cystic fibrosis patients chronically infected with Pseudomonadota.BMC pulmonary medicine · 2026Article
- Single-cell transcriptomics reveals lateral transfers of multiple functional genes from prokaryotes to free-living ciliated protists in detrital food webs.Marine life science & technology · 2026Article
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- Ecological and evolutionary dynamics of the oral microbiome across childhood.bioRxiv : the preprint server for biology · 2026Article
- Gut Morphological Structure-Microbial Characteristics inAnimals : an open access journal from MDPI · 2025Article
- Laser-assisted microbial culturomics.Nature communications · 2025Article
- Identifying essential genes inJournal of bacteriology · 2025Article
- Multi-Metagenome Analysis Unravels Community Collapse After Sampling and Hints the Cultivation Strategy of CPR Bacteria in Groundwater.Microorganisms · 2025Article
- Comparative genomics reveals genetic diversity and differential metabolic potentials of the species of Arachnia and suggests reclassification of Arachnia propionica E10012 (=NBRC_14587) as novel species.Archives of microbiology · 2025Article
- Categorization of Bacteria That Leak from Activated Sludge to Secondary Treated Water: Year-round Observations.Microbes and environments · 2025Article
- Human oral microbiome and its influence on mental health and brain disorders.AIMS microbiology · 2025Review
- The Oral Microbiome: A Key Determinant of Oral Health.Advances in experimental medicine and biology · 2025Review
- Structure and composition of early biofilms formed on dental implants are complex, diverse, subject-specific and dynamic.NPJ biofilms and microbiomes · 2024Article
- Revealing an unprecedented diversity of episymbiotic Saccharibacteria in a high-quality genome collection.NPJ biofilms and microbiomes · 2024Article
- Fusion/fission protein family identification in Archaea.mSystems · 2024Article
- Metagenomic analysis of healthy and diseased peri-implant microbiome under different periodontal conditions: a cross-sectional study.BMC oral health · 2024Article
- Omics for deciphering oral microecology.International journal of oral science · 2024Review
- Enrichment of different taxa of the enigmatic candidate phyla radiation bacteria using a novel picolitre droplet technique.ISME communications · 2024Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundThe vast majority of bacteria on earth have not yet been cultivated. There are many bacterial phyla with no cultivated examples including most members of the Candidate Phylum Radiation with the exception of human oral isolates from the phylum Saccharibacteria.
aimsThe aims of this research were to develop reproducible methods and validate approaches for the cultivation of human oral Saccharibacteria and to identify the conceptual pitfalls that delayed isolation of these bacteria for 20 years after their discovery.
methodsOral samples were dispersed and passed through 0.2 µm membrane filters. The ultrasmall saccharibacterial cells in the filtrate were pelleted, inoculated into broth cultures of potential bacterial host cells and passaged into fresh medium every 2-3 days.
resultsThirty-two isolates representing four species of Saccharibacteria were isolated in stable coculture with three species of host bacteria from the phylum
conclusionsHuman oral Saccharibacteria are obligate bacterial parasites that can be stably passaged in coculture with specific species of host bacteria. Isolating these important members of the human oral microbiome, and many natural environments, requires abandoning many of Koch's concepts and methods and embracing novel microbiological approaches.
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