ArticleScientific reports2020
Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Establishment of primary cell culture from deep-water fishes: an early insight.Fish physiology and biochemistry · 2026Article
- Cell bank system, establishment, and application in the virus research, diagnosis, and biopharmaceutical industries.Biotechnology notes (Amsterdam, Netherlands) · 2025Review
- Cell Identification, Characterization, and Documentation for Use in the Production of Biological Products.Archives of Razi Institute · 2024Review
- Development and characterization of a new muscle cell culture system from Clarias magur (Hamilton, 1822).Fish physiology and biochemistry · 2023Article
- Establishment and Characterization of Continuous Satellite Muscle Cells from Olive Flounder (Cells · 2023Article
- Development of a cell line from skeletal trunk muscle of the fishCytotechnology · 2023Article
- Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus).In vitro cellular & developmental biology. Animal · 2023Article
- Role and relevance of fish cell lines in advanced in vitro research.Molecular biology reports · 2022Review
- Reprocessing seafood waste: challenge to develop aquatic clean meat from fish cells.NPJ science of food · 2022Article
- Characterization of a macrophagic-like cell line derived from rabbit fish (Fish and shellfish immunology reports · 2021Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amphiprion ocellaris (ocellaris clownfish) is one of the most commercially important marine ornamental fish. A cell line designated as OCF was developed for the first time from the caudal fin of this fish species. The cell line was maintained in Leibovitz's-15 medium supplemented with 15% FBS (Fetal Bovine Serum) and was successfully subcultured up to 34 passages. The cell line was authenticated by sequencing mitochondrial cytochrome C oxidase subunit I (COI) and 16S rRNA genes. The growth rate of the OCF cell line was maximum in medium containing 20% FBS and 1% of 0.2 M NaCl at 28 °C. Chromosome analysis revealed 48 diploid chromosomes. The OCF cell line was transfected with the pMaxGFP plasmid vector with 7% efficiency and GFP expression was observed. The OCF cell line was used for testing nervous necrosis virus (NNV) susceptibility. Cytopathic effect (CPE) was observed in terms of plaque formation after virus inoculation. Nested PCR confirmed the susceptibility of the OCF cell line to NNV. The cell line was successfully cryopreserved by a slow freezing procedure at - 80 °C with a revival efficiency of 70-75%. The study revealed that the OCF cell line would be useful for virological studies. In addition, the cell line would play an important role as an in vitro tool for carrying out toxicological and biotechnological studies.
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