ArticleScientific reports2020
A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Disruption of chitin synthases impairs tick feeding and reproduction, validating a broad-spectrum acaricide target.Frontiers in cellular and infection microbiology · 2026Article
- Activation of the tick Toll pathway to control infection of Ixodes ricinus by the apicomplexan parasite Babesia microti.PLoS pathogens · 2024Article
- Characterisation of putative novel tick viruses and zoonotic risk prediction.Ecology and evolution · 2024Article
- Infection withViruses · 2022Article
- A proteomics informed by transcriptomics insight into the proteome of Ornithodoros erraticus adult tick saliva.Parasites & vectors · 2022Article
- Review
- Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions.Frontiers in cellular and infection microbiology · 2022Review
- Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine.Scientific reports · 2021Article
- Identification and Characterization of Immunodominant Proteins from Tick Tissue Extracts Inducing a Protective Immune Response againstVaccines · 2021Article
- Changing the Recipe: Pathogen Directed Changes in Tick Saliva Components.International journal of environmental research and public health · 2021Review
- Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection.Frontiers in cellular and infection microbiology · 2021Article
- Tick-Tattoo: DNA Vaccination AgainstFrontiers in immunology · 2021Article
- Identification of TickFrontiers in immunology · 2020Article
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Authors and funding
16 authors at 8 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ixodes ricinus is the vector for Borrelia afzelii, the predominant cause of Lyme borreliosis in Europe, whereas Ixodes scapularis is the vector for Borrelia burgdorferi in the USA. Transcription of several I. scapularis genes changes in the presence of B. burgdorferi and contributes to successful infection. To what extend B. afzelii influences gene expression in I. ricinus salivary glands is largely unknown. Therefore, we measured expression of uninfected vs. infected tick salivary gland genes during tick feeding using Massive Analysis of cDNA Ends (MACE) and RNAseq, quantifying 26.179 unique transcripts. While tick feeding was the main differentiator, B. afzelii infection significantly affected expression of hundreds of transcripts, including 465 transcripts after 24 h of tick feeding. Validation of the top-20 B. afzelii-upregulated transcripts at 24 h of tick feeding in ten biological genetic distinct replicates showed that expression varied extensively. Three transcripts could be validated, a basic tail protein, a lipocalin and an ixodegrin, and might be involved in B. afzelii transmission. However, vaccination with recombinant forms of these proteins only marginally altered B. afzelii infection in I. ricinus-challenged mice for one of the proteins. Collectively, our data show that identification of tick salivary genes upregulated in the presence of pathogens could serve to identify potential pathogen-blocking vaccine candidates.
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