ArticlePloS one2020
Sensitivity evaluation of 2019 novel coronavirus (SARS-CoV-2) RT-PCR detection kits and strategy to reduce false negative.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 64 citations in OpenAlex.
- Comprehensive performance evaluation of a high-throughput automated system for pathogen nucleic acid detection in clinical settings.Frontiers in microbiology · 2025Article
- COMPARISON OF ABBOTT REAL TIME SARS-COV-2 ASSAY AND LIFERIVER NOVEL CORONAVIRUS REAL TIME MULTIPLEX KIT FOR THE RT-PCR BASED DETECTION OF SARS-COV-2 FROM NASOPHARYNGEAL SWABS.Acta clinica Croatica · 2024Article
- Evaluations of modes of pooling specimens for COVID-19 screened by quantitative PCR and droplet digital PCR.Scientific reports · 2024Article
- Point-of-Care Devices for Viral Detection: COVID-19 Pandemic and Beyond.Micromachines · 2023Review
- 'CoviSwiftJournal of virological methods · 2023Article
- Screening and confirmation tests for SARS-CoV-2: benefits and drawbacks.Beni-Suef University journal of basic and applied sciences · 2023Review
- Evaluation of Various Alternative Economical and High Throughput SARS-CoV-2 Testing Methods within Resource-Limited Settings.International journal of molecular sciences · 2022Article
- Considerations for the selection of tests for SARS-CoV-2 molecular diagnostics.Molecular biology reports · 2022Review
- PCovNet: A presymptomatic COVID-19 detection framework using deep learning model using wearables data.Computers in biology and medicine · 2022Article
- Microfluidics-Based POCT for SARS-CoV-2 Diagnostics.Micromachines · 2022Review
- MicroRNA of N-region from SARS-CoV-2: Potential sensing components for biosensor development.Biotechnology and applied biochemistry · 2022Article
- Factors associated with weak positive SARS-CoV-2 diagnosis by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR).Pathology · 2022Article
- The Matrix Effect in the RT-PCR Detection of SARS-CoV-2 Using Saliva without RNA Extraction.Diagnostics (Basel, Switzerland) · 2022Article
- Application experience of a rapid nucleic acid detection system for COVID-19.Microbes and infection · 2022Article
- Covid-19 detection in chest X-ray through random forest classifier using a hybridization of deep CNN and DWT optimized features.Journal of King Saud University. Computer and information sciences · 2022Article
- Comparative Diagnostic Accuracy of the STANDARD M10 Assay for the Molecular Diagnosis of SARS-CoV-2 in the Point-of-Care and Critical Care Settings.Journal of clinical medicine · 2022Article
- Review
- COVID-19 PCR test performance on samples stored at ambient temperature.Journal of virological methods · 2022Article
- Multiplex real-time RT-PCR method for the diagnosis of SARS-CoV-2 by targeting viral N, RdRP and human RP genes.Scientific reports · 2022Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The early detection and differential diagnosis of respiratory infections increase the chances for successful control of COVID-19 disease. The nucleic acid RT-PCR test is regarded as the current standard for molecular diagnosis. However, the maximal specificity confirmation target ORF1ab gene is considered to be less sensitive than other targets in clinical application. In addition, recent evidence indicated that the initial missed diagnosis of asymptomatic patients with SARS-CoV-2 and discharged patients with "re-examination positive" might be due to low viral load, and the ability of rapid mutation of SARS-CoV-2 also increases the rate of false-negative results. Moreover, the mixed sample nucleic acid detection is helpful in seeking out the early community transmission of SARS-CoV-2 rapidly, but the detection kit needs ultra-high detection sensitivity. Herein, the lowest detection concentration of different nucleic acid detection kits was evaluated and compared to provide direct evidence for the selection of kits for mixed sample detection or make recommendations for the selection of validation kit, which is of great significance for the prevention and control of the current epidemic and the discharge criteria of low viral load patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.