ArticlePLoS neglected tropical diseases2020
Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
- Virulence profiling and biofilm-forming capacity of differentVeterinary and animal science · 2026Article
- Multidrug resistance, biofilm formation, and pathotype diversity of Escherichia coli isolated from diarrheic lambs in intensive sheep farms, Aksu, Xinjiang, China.BMC microbiology · 2026Article
- Genomic features of three major diarrhoeagenicMicrobial genomics · 2025Article
- Integrating procurement, prescription, and resistance data to strengthen antimicrobial stewardship: insights from a public health institution in India.Frontiers in microbiology · 2025Article
- Prevalence of CTX-M types among ESBL-producing pathogenicFood science and biotechnology · 2024Article
- Article
- Antimicrobial resistance in food-borne pathogens at the human-animal interface: Results from a large surveillance study in India.One health (Amsterdam, Netherlands) · 2024Article
- Association of Putative Virulence Genes with HEp-2 Cell Adherence and Biofilm Production in EnteroaggregativeIndian journal of microbiology · 2024Article
- Dynamic Interactions between Diarrhoeagenic EnteroaggregativeMicroorganisms · 2023Article
- A Mini-Review of EnteroaggregativePolish journal of microbiology · 2023Review
- Multidrug resistance pattern and molecular epidemiology of pathogens among children with diarrhea in Bangladesh, 2019-2021.Scientific reports · 2023Article
- Prevalence and Antimicrobial Resistance Profile of DiarrheagenicAntibiotics (Basel, Switzerland) · 2023Article
- Are non-lactose-fermentingAccess microbiology · 2023Article
- Phenotypic and genotypic characteristics ofFrontiers in public health · 2023Article
- Prevalence of Virulence Genes AmongJournal of veterinary research · 2022Article
- A Novel Adult Murine Model of Typical EnteroaggregativeFrontiers in cellular and infection microbiology · 2022Article
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Authors and funding
19 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteroaggregative Escherichia coli (EAEC) is an evolving enteric pathogen that causes acute and chronic diarrhea in developed and industrialized nations in children. EAEC epidemiology and the importance of atypical EAEC (aEAEC) isolation in childhood diarrhea are not well documented in the Indian setting. A comparative analysis was undertaken to evaluate virulence, phylogeny, and antibiotic sensitivity among typical tEAEC versus aEAEC. A total of 171 EAEC isolates were extracted from a broad surveillance sample of diarrheal (N = 1210) and healthy children (N = 550) across North India. Polymerase chain reaction (PCR) for the aggR gene (master regulator gene) was conducted to differentiate tEAEC and aEAEC. For 21 virulence genes, we used multiplex PCR to classify possible virulence factors among these strains. Phylogenetic classes were identified by a multiplex PCR for chuA, yjaA, and a cryptic DNA fragment, TspE4C2. Antibiotic susceptibility was conducted by the disc diffusion method as per CLSI guidelines. EAEC was associated with moderate to severe diarrhea in children. The prevalence of EAEC infection (11.4%) was higher than any other DEC group (p = 0.002). tEAEC occurrence in the diarrheal group was higher than in the control group (p = 0.0001). tEAEC strain harbored more virulence genes than aEAEC. astA, aap, and aggR genes were most frequently found in the EAEC from the diarrheal population. Within tEAEC, this gene combination was present in more than 50% of strains. Also, 75.8% of EAEC strains were multidrug-resistant (MDR). Phylogroup D (43.9%) and B1 (39.4%) were most prevalent in the diarrheal and control group, respectively. Genetic analysis revealed EAEC variability; the comparison of tEAEC and aEAEC allowed us to better understand the EAEC virulence repertoire. Further microbiological and epidemiological research is required to examine the pathogenicity of not only typical but also atypical EAEC.
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