Evidence map›Paper›PMID 33205850›Full record

ArticleMedical and veterinary entomology2021

Geometric morphometrics to distinguish the cryptic species Anopheles minimus and An. harrisoni in malaria hot spot villages, western Thailand.

K Chatpiyaphat, S Sumruayphol, J-P Dujardin, Y Samung, A Phayakkaphon, L Cui, J Ruangsittichai, S Sungvornyothin, J Sattabongkot, P Sriwichai

Abstract read
In one paragraph

Article in Medical and veterinary entomology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Validation of wing geometric morphometrics inFrontiers in insect science · 2025
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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

K ChatpiyaphatDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
S SumruaypholDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
J-P DujardinUnité Mixte de Recherche 177-Interactions Hôte-Vecteur-Parasite-Enironnement dans les Maladies Tropicales Négligées dues aux Trypanosomatidés, Centre International de Recherches Agronomiques pour le Développement (CIRAD), Institut de Recherches pour le Développement (IRD), Campus international de Baillarguet, Montpellier, France.
Y SamungDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
A PhayakkaphonDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
L CuiDepartment of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, U.S.A.
J RuangsittichaiDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
S SungvornyothinDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
J SattabongkotVivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
P SriwichaiDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Funding

Southeast Asia Malaria Research CenterU19AI089672 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI CUI, LIWANG · 2010 to 2023
$28.9M
Vivax Malaria culture technique and genetic diversity in ThailandD43TW006571 · FIC · PENNSYLVANIA STATE UNIVERSITY-UNIV PARK · PI CUI, LIWANG · 2004 to 2018
$1.9M
FIC NIH HHS D43 TW006571NIAID NIH HHS U19 AI089672
6 · The paper itself

Abstract

Anopheles minimus Theobald 1901 and An. harrisoni Harbach & Manguin 2007 belong to the same species complex. They are morphologically similar and can exist in sympatry but have blood host preferences. The most accurate method for their identification is based on molecular techniques. Here, we measure the level of interspecific discrimination by geometric morphometry. Sixty-seven An. minimus and 22 An. harrisoni specimens were selected based on their morphological integrity and confirmed by identification polymerase chain reaction of internal transcribed spacer 2. These samples were used as reference data allowing for a morphometric identification based on geometric shape. Despite size overlap between the two species, there was a significant shape divergence allowing for differentiation of An. minimus and An. harrisoni with 90% accuracy. An intraspecific study of An. minimus showed a summer period associated to the reducing of wing size, which did not influence the shape-based differentiation of An. harrisoni. Wing venation geometry can be used to distinguish between these cryptic species mainly based on shaped divergence. This study suggests that geometric morphometrics represent a convenient low-cost method to complement morphological identification, especially concerning damaged specimens, i.e., insects having accidentally lost the anatomical features allowing a reliable morphological identification.

Indexed as

AnophelesMalariaAnimalsBase SequencePolymerase Chain ReactionThailandAn. minimusAnopheles harrisonigeometric morphometricspecies complex

Identifiers

PMID33205850
PMCPMC8451769

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.