ArticleMethodsX2020
Optimising total RNA quality and quantity by phenol-chloroform extraction method from human visceral adipose tissue: A standardisation study.
Article in MethodsX, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- Whole-genome survey information revealed the genome characteristics, mitochondrial genome diversity and evolutionary patterns of Stomatopoda species.BMC genomics · 2025Article
- Therapeatic evaluation and single cell analysis of adipose stromal vascular fraction isolation from a commercial cell separation system.Stem cell research & therapy · 2025Article
- An ovine model shows that subcutaneous adipose tissue fibrosis occurs early in polycystic ovary syndrome (PCOS).Journal of molecular endocrinology · 2025Article
- Neuroprotective Effects of Tectoridin in HNeurochemical research · 2025Article
- The Protective Effect of Taurine and Curcumin on Autophagy-Related Genes in the Oocytes of the Mouse Treated with Acrylamide.Iranian journal of medical sciences · 2025Article
- Isolation of native microorganisms from Shengli lignite and study on their ability to dissolve lignite.Bioprocess and biosystems engineering · 2024Article
- The effect of GnRH-a on the angiogenesis of endometriosis.Hormones (Athens, Greece) · 2024Article
- Wound fluid sampling methods and analysis of cytokine mRNA expression in ulcers from patients with diabetes mellitus.Regenerative therapy · 2024Review
- A comparison of five methods to maximize RNA and DNA isolation yield from adipose tissue.PeerJ · 2024Article
- Fabrication of Injectable Kartogenin-Conjugated Composite Hydrogel with a Sustained Drug Release for Cartilage Repair.Pharmaceutics · 2023Article
- Comparison of Four Methods of RNA Extraction and cDNA Synthesis from The Venom of Peruvian Snakes of the GenusInternational journal of molecular sciences · 2023Article
- The Caenorhabditis elegans anchor cell transcriptome: ribosome biogenesis drives cell invasion through basement membrane.Development (Cambridge, England) · 2023Article
- Analyzing the Association of Visceral Adipose Tissue Growth Differentiation Factor-15 and MicroRNA in Type 2 Diabetes Mellitus.Journal of obesity & metabolic syndrome · 2023Article
- Article
- Physical Activity and Exercise Promote Peroxisome Proliferator-Activated Receptor Gamma Expression in Adipose Tissues of Obese Adults.Iranian journal of public health · 2022Article
- The Effect of Tropical Temperatures on the Quality of RNA Extracted from Stabilized Whole-Blood Samples.International journal of molecular sciences · 2022Article
- Prevalence, Diversity, and Risk Factors for Cervical HPV Infection in Women Screened for Cervical Cancer in Belém, Pará, Northern Brazil.Pathogens (Basel, Switzerland) · 2022Article
- FOXM1 mediates GDF-15 dependent stemness and intrinsic drug resistance in breast cancer.Molecular biology reports · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multitude of challenges is faced during RNA extraction from human visceral adipose tissue (VAT) due to its atypical nature and a dearth of existing literature. Our study provides a convenient and inexpensive manual method using TRIzol reagent for the reproducible recovery of intact RNA from sparse human VAT samples. Fifty-two (52) samples were grouped and tested for the effect of different factors viz. initial VAT amount, TRIzol volume per unit tissue mass, residual fat following homogenisation and first centrifugation, an additional chloroform wash, and an additional ethanol wash on the extraction process. We found that increasing initial tissue mass and decreasing TRIzol volume simultaneously improved RNA yield and purity. A fat layer removal step and additional ethanol wash further propel the A260/280 and A260/230 to their desired values. Our modifications in the isolation protocol were combined and tested through reverse transcriptase quantitative PCR, which yielded consistent results, upholding our optimisation.
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