ArticleArthritis research & therapy2020
Widespread regulation of gene expression by glucocorticoids in chondrocytes from patients with osteoarthritis as determined by RNA-Seq.
Article in Arthritis research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Triamcinolone Modulates Chondrocyte Biomechanics and Calcium-Dependent Mechanosensitivity.International journal of molecular sciences · 2026Article
- Dexamethasone regulates gene expression in chondrocytes through MKP-1 and downregulates cholesterol hydroxylases CH25H and CYP7B1.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025Article
- Dexamethasone: a double-edged sword in the treatment of osteoarthritis.Scientific reports · 2025Article
- The pivotal role of the Hes1/Piezo1 pathway in the pathophysiology of glucocorticoid-induced osteoporosis.JCI insight · 2024Article
- The role of targeting glucose metabolism in chondrocytes in the pathogenesis and therapeutic mechanisms of osteoarthritis: a narrative review.Frontiers in endocrinology · 2024Review
- ER stress-induced YAP upregulation leads to chondrocyte phenotype loss in age-related osteoarthritis.Frontiers in pharmacology · 2024Article
- Advances in understanding effects of miRNAs on apoptosis, autophagy, and pyroptosis in knee osteoarthritis.Molecular genetics and genomics : MGG · 2023Review
- The Combination of Glucocorticoids and Hyaluronic Acid Enhances Efficacy in IL-1β/IL-17-Treated Bovine Osteochondral Grafts Compared with Individual Application.International journal of molecular sciences · 2023Article
- MKP-1 Deficiency Exacerbates Skin Fibrosis in a Mouse Model of Scleroderma.International journal of molecular sciences · 2023Article
- NFATc1 Regulation of Dexamethasone-InducedCells · 2023Article
- Epigenetics as a Therapeutic Target in Osteoarthritis.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Dexamethasone-Induced Fatty Acid Oxidation and Autophagy/Mitophagy Are Essential for T-ALL Glucocorticoid Resistance.Cancers · 2023Article
- Identification of Key Diagnostic Markers and Immune Infiltration in Osteoarthritis.Combinatorial chemistry & high throughput screening · 2023Article
- Transcriptomic changes in porcine articular cartilage one year following disruption of the anterior cruciate ligament.PloS one · 2023Article
- Dexamethasone Attenuates the Expression of MMP-13 in Chondrocytes through MKP-1.International journal of molecular sciences · 2022Article
- Mitochondria and sensory processing in inflammatory and neuropathic pain.Frontiers in pain research (Lausanne, Switzerland) · 2022Review
- Nfib promotes chondrocyte proliferation and inhibits differentiation by mildly regulating Sox9 and its downstream genes.Molecular biology reports · 2021Article
- Small Noncoding RNAs in Knee Osteoarthritis: The Role of MicroRNAs and tRNA-Derived Fragments.International journal of molecular sciences · 2021Review
- Glucocorticoids are double-edged sword in the treatment of COVID-19 and cancers.International journal of biological sciences · 2021Review
- Zebrafish models for glucocorticoid-induced osteoporosis.Tzu chi medical journalReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIntra-articular glucocorticoid (GC) injections are widely used as a symptomatic treatment for osteoarthritis (OA). However, there are also concerns about their potentially harmful effects, and their detailed effects on chondrocyte phenotype remain poorly understood.
methodsWe studied the effects of dexamethasone on gene expression in OA chondrocytes with RNA-Seq. Chondrocytes were isolated from the cartilage from OA patients undergoing knee replacement surgery and cultured with or without dexamethasone for 24 h. Total RNA was isolated and sequenced, and functional analysis was performed against the Gene Ontology (GO) database. Results for selected genes were confirmed with RT-PCR. We also investigated genes linked to OA in recent genome-wide expression analysis (GWEA) studies.
resultsDexamethasone increased the expression of 480 and reduced that of 755 genes with a fold change (FC) 2.0 or greater. Several genes associated with inflammation and cartilage anabolism/catabolism as well as lipid and carbohydrate metabolism were among the most strongly affected genes. In the GO analysis, genes involved in the extracellular matrix organization, cell proliferation and adhesion, inflammation, and collagen synthesis were enriched among the significantly affected genes. In network analysis, NGF, PI3KR1, and VCAM1 were identified as central genes among those most strongly affected by dexamethasone.
conclusionsThis is the first study investigating the genome-wide effects of GCs on the gene expression in OA chondrocytes. In addition to clear anti-inflammatory and anticatabolic effects, GCs affect lipid and glucose metabolism in chondrocytes, an observation that might be particularly important in the metabolic phenotype of OA.
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