Evidence map›Paper›PMID 33202599›Full record

ArticleMicroorganisms2020

A Reporter System for Fast Quantitative Monitoring of Type 3 Protein Secretion in Enteropathogenic

Luit Barkalita, Athina G Portaliou, Maria S Loos, Biao Yuan, Spyridoula Karamanou, Anastassios Economou

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Luit BarkalitaRega Institute for Medical Research, Laboratory of Molecular Bacteriology, Department of Microbiology, Immunology and Transplantation, KU Leuven, B-3000 Leuven, Belgium.ORCID 0000-0001-6113-3649
Athina G PortaliouRega Institute for Medical Research, Laboratory of Molecular Bacteriology, Department of Microbiology, Immunology and Transplantation, KU Leuven, B-3000 Leuven, Belgium.
Maria S LoosRega Institute for Medical Research, Laboratory of Molecular Bacteriology, Department of Microbiology, Immunology and Transplantation, KU Leuven, B-3000 Leuven, Belgium.
Biao YuanRega Institute for Medical Research, Laboratory of Molecular Bacteriology, Department of Microbiology, Immunology and Transplantation, KU Leuven, B-3000 Leuven, Belgium.
Spyridoula KaramanouRega Institute for Medical Research, Laboratory of Molecular Bacteriology, Department of Microbiology, Immunology and Transplantation, KU Leuven, B-3000 Leuven, Belgium.
Anastassios EconomouRega Institute for Medical Research, Laboratory of Molecular Bacteriology, Department of Microbiology, Immunology and Transplantation, KU Leuven, B-3000 Leuven, Belgium.ORCID 0000-0002-1770-507X
Rega Institute for Medical Research · BE

Funding

Fonds Wetenschappelijk Onderzoek G002516NFonds Wetenschappelijk Onderzoek G0H2116N
6 · The paper itself

Abstract

The type 3 secretion system is essential for pathogenesis of several human and animal Gram-negative bacterial pathogens. The T3SS comprises a transmembrane injectisome, providing a conduit from the bacterial cytoplasm to the host cell cytoplasm for the direct delivery of effectors (including toxins). Functional studies of T3SS commonly monitor the extracellular secretion of proteins by SDS-PAGE and western blot analysis, which are slow and semi-quantitative in nature. Here, we describe an enzymatic reporter-based quantitative and rapid in vivo assay for T3SS secretion studies in enteropathogenic

Indexed as

alkaline phosphatase activityEPECquantitative in vivo secretion assaySctA-PhoAType 3 secretion

Identifiers

PMID33202599
PMCPMC7696366
OpenAlexW3102147795

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.