Evidence map›Paper›PMID 33201379›Full record

ArticleGlycoconjugate journal2020

New approach to prepare fluorogenic branched dextrins for assaying glycogen debranching enzyme.

Miyu Sakaguchi, Yasushi Makino, Hiroshi Matsubara

Abstract read
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In one paragraph

Article in Glycoconjugate journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Miyu SakaguchiDepartment of Chemistry, Graduate School of Science, Osaka Prefecture University, Gakuen-cho 1-1, Naka-ku, Sakai Osaka, 599-8531, Osaka, Japan.
Yasushi MakinoDepartment of Chemistry, Graduate School of Science, Osaka Prefecture University, Gakuen-cho 1-1, Naka-ku, Sakai Osaka, 599-8531, Osaka, Japan. ymakino@c.s.osakafu-u.ac.jp.ORCID 0000-0002-8827-7412
Hiroshi MatsubaraDepartment of Chemistry, Graduate School of Science, Osaka Prefecture University, Gakuen-cho 1-1, Naka-ku, Sakai Osaka, 599-8531, Osaka, Japan.ORCID 0000-0003-3224-4336
Osaka Prefecture University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glycogen debranching enzyme (GDE), together with glycogen phosphorylase (GP), is responsible for the complete degradation of glycogen. GDE has distinct catalytic sites for 4-α-glucanotransferase and amylo-α-1,6-glucosidase. For the GDE sensitive assay, we previously developed the GP limit fluorogenic branched dextrin Glcα1-4Glcα1-4Glcα1-4Glcα1-4(Glcα1-4Glcα1-4Glcα1-4Glcα1-6)Glcα1-4Glcα1-4Glcα1-4GlcPA (B4/84, where Glc = D-glucose and GlcPA = 1-deoxy-1-[(2-pyridyl)amino]-D-glucitol). However, B4/84 is not widely available because of difficulties in its chemical synthesis and positional-isomer separation (0.33% yield by α-1,6-coupling of maltotetraose with Glc

Indexed as

Binding SitesChromatography, High Pressure LiquidDextrinsFluorescent DyesGlucosyltransferasesGlycogenGlycogen Debranching Enzyme SystemGlycogen PhosphorylaseHumansLiverOligosaccharides4 alpha-glucanotransferasecyclomaltodextrin glucanotransferaseDextrinsFluorescent DyesGlucosyltransferasesGlycogenGlycogen Debranching Enzyme SystemGlycogen PhosphorylaseOligosaccharidesFluorogenic branched dextrinGlycogen debranching enzymeGlycogen storage disease type IIIPhosphorylase limit dextrinPyridylamination

Identifiers

PMID33201379
OpenAlexW3101585593

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.