Evidence map›Paper›PMID 33200852›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2020

A Two-Armed Probe for In-Cell DEER Measurements on Proteins*.

Qing Miao, Enrico Zurlo, Donny de Bruin, Joeri A J Wondergem, Monika Timmer, Anneloes Blok, Doris Heinrich, Mark Overhand, Martina Huber, Marcellus Ubbink

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. A Two-Armed Probe for In-Cell DEER Measurements on Proteins*.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Qing MiaoLeiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
Enrico ZurloDepartment of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, PO box 9504, 2300, RA, Leiden, The Netherlands.
Donny de BruinDepartment of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, PO box 9504, 2300, RA, Leiden, The Netherlands.
Joeri A J WondergemDepartment of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, PO box 9504, 2300, RA, Leiden, The Netherlands.
Monika TimmerLeiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
Anneloes BlokLeiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
Doris HeinrichDepartment of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, PO box 9504, 2300, RA, Leiden, The Netherlands.ORCID http://orcid.org/0000-0001-9485-7141
Mark OverhandLeiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
Martina HuberDepartment of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, PO box 9504, 2300, RA, Leiden, The Netherlands.ORCID http://orcid.org/0000-0001-7632-1968
Marcellus UbbinkLeiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.ORCID http://orcid.org/0000-0002-2615-6914

Funding

Chinese Scholarship Council CSC grant to Q. M, No. 201506870013Fraunhofer Society Fraunhofer Attract grant "3DNanoCell"Fraunhofer Society Fraunhofer ICON grant "BioSensing"Nederlandse Organisatie voor Wetenschappelijk Onderzoek Grant No. 711.014.003Nederlandse Organisatie voor Wetenschappelijk Onderzoek Spinoza Prijs 2014 for Prof. Dirk Bouwmeester
6 · The paper itself

Abstract

The application of double electron-electron resonance (DEER) with site-directed spin labeling (SDSL) to measure distances in proteins and protein complexes in living cells puts rigorous restraints on the spin-label. The linkage and paramagnetic centers need to resist the reducing conditions of the cell. Rigid attachment of the probe to the protein improves precision of the measured distances. Here, three two-armed Gd

Indexed as

DictyosteliumGadoliniumElectron Spin Resonance SpectroscopyProteinsSpin LabelsGadoliniumProteinsSpin Labelsdouble electron-electron resonance (DEER)EPR spectroscopygadoliniumprotein structuresspin labels

Identifiers

PMID33200852
PMCPMC7839491

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.