ArticleBioscience reports2020
Acetylation/deacetylation and microtubule associated proteins influence flagellar axonemal stability and sperm motility.
Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Cryopreservation of Human Spermatozoa Using Penetrating Cryoprotectant-Free Vitrification Medium Exhibits an Altered Proteomic Profile Compared to the Conventional Rapid Freezing Method.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Decoding Infertility: the Epigenetic Influence of HDACs on Reproductive Function.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- The dynamics of ciliogenesis in prepubertal mouse meiosis reveals new clues about testis maturation.Frontiers in cell and developmental biology · 2026Article
- Genetic and epigenetic landscape of male infertility.Trends in genetics : TIG · 2025Review
- Unveiling the reproductive risks of PFOS: testicular proteome disruption and hormonal imbalance in male rats†.Biology of reproduction · 2025Article
- Impact of Type 1 Diabetes on Testicular Microtubule Dynamics, Sperm Physiology, and Male Reproductive Health in Rat.International journal of molecular sciences · 2025Article
- Searching for the 'X' factor: investigating the genetics of primary ovarian insufficiency.Journal of ovarian research · 2024Review
- TMEM232 is required for the formation of sperm flagellum and male fertility in mice.Cell death & disease · 2024Article
- Scriptaid is a prospective agent for improving human asthenozoospermic sample quality and fertilization rate in vitro.Asian journal of andrology · 2024Article
- Metabolic profiling identifies Qrich2 as a novel glutamine sensor that regulates microtubule glutamylation and mitochondrial function in mouse sperm.Cellular and molecular life sciences : CMLS · 2024Article
- Abnormal centriolar biomarker ratios correlate with unexplained bull artificial insemination subfertility: a pilot study.Scientific reports · 2023Article
- [Regulatory effects of bio-intensity electric field on microtubule acetylation in human epidermal cell line HaCaT].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2022Article
- Transcriptome Analysis Reveals Key Gene Expression Changes in Blue Catfish Sperm in Response to Cryopreservation.International journal of molecular sciences · 2022Article
- Tubulin posttranslational modifications modify the atypical spermatozoon centriole.microPublication biology · 2022Article
- Article
- Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function.Clinical epigenetics · 2021Article
- Molecular basis of the morphogenesis of sperm head and tail in mice.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PTMs and microtubule-associated proteins (MAPs) are known to regulate microtubule dynamicity in somatic cells. Reported literature on modulation of α-tubulin acetyl transferase (αTAT1) and histone deacetylase 6 (HDAC6) in animal models and cell lines illustrate disparity in correlating tubulin acetylation status with stability of MT. Our earlier studies showed reduced acetyl tubulin in sperm of asthenozoospermic individuals. Our studies on rat sperm showed that on inhibition of HDAC6 activity, although tubulin acetylation increased, sperm motility was reduced. Studies were therefore undertaken to investigate the influence of tubulin acetylation/deacetylation on MT dynamicity in sperm flagella using rat and human sperm. Our data on rat sperm revealed that HDAC6 specific inhibitor Tubastatin A (T) inhibited sperm motility and neutralized the depolymerizing and motility debilitating effect of Nocodazole. The effect on polymerization was further confirmed in vitro using pure MT and recHDAC6. Also polymerized axoneme was less in sperm of asthenozoosperm compared to normozoosperm. Deacetylase activity was reduced in sperm lysates and axonemes exposed to T and N+T but not in axonemes of sperm treated similarly suggesting that HDAC6 is associated with sperm axonemes or MT. Deacetylase activity was less in asthenozoosperm. Intriguingly, the expression of MDP3 physiologically known to bind to HDAC6 and inhibit its deacetylase activity remained unchanged. However, expression of acetyl α-tubulin, HDAC6 and microtubule stabilizing protein SAXO1 was less in asthenozoosperm. These observations suggest that MAPs and threshold levels of MT acetylation/deacetylation are important for MT dynamicity in sperm and may play a role in regulating sperm motility.
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