ArticleThe Journal of cell biology1987
Incorporation of nascent myosin heavy chains into thick filaments of cardiac myocytes in thyroid-treated rabbits.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Thick filament-associated myosin undergoes frequent replacement at the tip of the thick filament.FEBS open bio · 2022Article
- Random myosin loss along thick-filaments increases myosin attachment time and the proportion of bound myosin heads to mitigate force decline in skeletal muscle.Archives of biochemistry and biophysics · 2014Article
- Skeletal muscle myofilament adaptations to aging, disease, and disuse and their effects on whole muscle performance in older adult humans.Frontiers in physiology · 2014Review
- Cardiomyopathy mutations in the tail of β-cardiac myosin modify the coiled-coil structure and affect integration into thick filaments in muscle sarcomeres in adult cardiomyocytes.The Journal of biological chemistry · 2013Article
- Resistance training alters skeletal muscle structure and function in human heart failure: effects at the tissue, cellular and molecular levels.The Journal of physiology · 2012Article
- Determination of rate constants for turnover of myosin isoforms in rat myocardium: implications for in vivo contractile kinetics.American journal of physiology. Heart and circulatory physiology · 2009Article
- Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells.The Journal of cell biology · 2001Article
- Sarcomeric gene expression and contractility in myofibroblasts.The Journal of cell biology · 1997Article
- Thyroid hormone effects on contractility and myosin composition of soleus muscle and single fibres from young and old rats.The Journal of physiology · 1996Article
- Distribution of developmental myosin isoforms in isolated A-segments.Journal of muscle research and cell motility · 1992Article
- Visualization of myosin exchange between synthetic thick filaments.Journal of muscle research and cell motility · 1991Article
- Cell-free incorporation of newly synthesized myosin subunits into thick myofilaments.Journal of muscle research and cell motility · 1991Article
- Myosin mRNA accumulation and myofibrillogenesis at the myotendinous junction of stretched muscle fibers.The Journal of cell biology · 1990Article
- Subcellular localization of newly incorporated myosin in rabbit fast skeletal muscle undergoing stimulation-induced type transformation.Journal of muscle research and cell motility · 1990Article
- Differential distribution of myosin isoforms among the myofibrils of individual developing muscle fibers.The Journal of cell biology · 1990Article
- Distribution of fast myosin heavy chain isoforms in thick filaments of developing chicken pectoral muscle.The Journal of cell biology · 1989Article
- Association of microinjected myosin and its subfragments with myofibrils in living muscle cells.The Journal of cell biology · 1988Article
- Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin.The Journal of cell biology · 1988Article
- Feasibility of long-term storage of graded information by the Ca2+/calmodulin-dependent protein kinase molecules of the postsynaptic density.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Genetic analysis of myosin assembly in Caenorhabditis elegans.Molecular neurobiologyReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A monoclonal antibody (mAb 37) specific for alpha-myosin heavy chain (alpha-MHC) is used to follow the spatial and temporal incorporation of alpha-MHC into rabbit left ventricular myocytes. The expression of the two adult cardiac MHC genes, alpha and beta, is regulated by manipulating the thyroid hormone level of the animal. 10 wk on a propylthiouracil diet down-regulates expression of alpha-MHC to near 0%. alpha-MHC gene expression is up-regulated by injecting L-triiodothyronine (100 micrograms/kg per d) for 1-4 d. This protocol provides a means by which to follow the redistribution pattern of alpha-MHC within the myocyte in vivo. A uniform distribution of immunofluorescent signal is seen within every myocyte throughout the left ventricle. Ultracryomicrotomy without fixation is used to obtain sections for immunogold-electron microscopy. To quantify the immunogold method the density of gold-labeled antibody per unit of area tissue is determined for various regions of the sarcomere. Tissue from normal and 2-wk baby has a uniform distribution of gold density along the length of the A band. The average gold density of the A band increases with days of thyroid injection from 38 +/- 4 grains/micron 2 (n = 2 animals) (mean +/- SE) at day 1 to 182 +/- 59 grains (n = 2 animals) at day 4. There is a nonuniform incorporation of the newly synthesized alpha-MHC within the A band of thyroid-treated animals since 50% more of the alpha-MHC is found at the end of the A band while the center of the A band has 40% less than the average alpha-MHC content (grains/micron 2, n = 7 animals). These results support a thick filament assembly model that allows every myosin in a thick filament to be exchanged with new myosin. However, in the intact functioning myocyte, there is greater exchange of new myosin at the ends than in the central region of the thick filament.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.