Evidence map›Paper›PMID 33200314›Full record

ArticleMolecular biology reports2020

Effect of the Rho GTPase inhibitor-1 on the entry of dengue serotype 2 virus into EAhy926 cells.

Dongying Fan, Na Wu, Jing Zhang, Zhaoyang Wang, Peigang Wang, Na Gao, Jing An

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Immune Functions of Astrocytes in Viral Neuroinfections.International journal of molecular sciences · 2023
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Dongying FanDepartment of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.
Na WuDepartment of Experimental Animal, Capital Medical University, Beijing, 100069, China.
Jing ZhangMedical Research Center, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.
Zhaoyang WangDepartment of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.
Peigang WangDepartment of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.
Na GaoDepartment of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China. gao_na@ccmu.edu.cn.ORCID http://orcid.org/0000-0002-2941-1925
Jing AnDepartment of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.
Capital Medical University · CNBeijing Tongren Hospital · CN

Funding

National Natural Science Foundation of China 81671971National Natural Science Foundation of China 81871641National Natural Science Foundation of China U1602223Scientific Research Plan of the Beijing Municipal Education Committee KM201710025002
6 · The paper itself

Abstract

Dengue virus (DV) is the most rapidly spreading arbovirus in the world. Our previous studies indicated that Rac1, a kind of Rho GTPase, was related with the increased vascular permeability in DV infection. However, the molecular mechanisms that regulate the activity of the Rac1 pathway during DV infection is not fully understood yet. Recently, Rho-specific guanine nucleotide dissociated inhibitors (Rho GDIs), as a pivotal upstream regulator of Rho GTPase, attract our attention. To identify the role of GDI-1 in DV2 infection, the expression of GDI in Eahy926 cells was detected. Moreover, a GDI-1 down-regulated cell line was constructed to explore the correlation between GDI-1 and Rac1 and to further evaluate the function of GDI in DV life cycle. Our results indicated that DV2 infection could up-regulate GDI-1 expression, and down-regulation of GDI enhanced the activity of Rac1. In addition, down-regulated GDI-1 significantly inhibited all steps of DV2 replication cycle. GDI-1 plays an important role in DV2 infection via negatively regulating the activation of the Rac1-actin pathway. These results not only contribute to our further understanding of the pathogenesis of severe dengue but also provide further insight into the development of antiviral drugs.

Indexed as

ActinsAedesAnimalsCell LineDengueDengue VirusHumansrac1 GTP-Binding Proteinrho Guanine Nucleotide Dissociation Inhibitor alphaVirus InternalizationVirus ReplicationActinsARHGDIA protein, humanrac1 GTP-Binding ProteinRAC1 protein, humanrho Guanine Nucleotide Dissociation Inhibitor alphaDengue virusEAhy926 cellsGuanine nucleotide dissociated inhibitor

Identifiers

PMID33200314
OpenAlexW3103054899

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.