ArticleNature protocols2020
An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity.
Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Spatiotemporal regulation of DNA repair proteins between Golgi and nucleus maintains genome stability.The Journal of cell biology · 2026Article
- Towards a validation of the standard and enzyme-linked comet assay: a retrospective variability analysis.Archives of toxicology · 2026Review
- Agent-specific DNA repair kinetic in 3T3 cells: a comparative study using the in vitro comet assay.Toxicology research · 2025Article
- DNA damage in oral mucosal epithelial cells cultured in complex and xenobiotic-free media: a comparison study.Mutagenesis · 2025Article
- A pooled analysis of host factors that affect nucleotide excision repair in humans.Mutagenesis · 2025Article
- RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis.Nature communications · 2024Article
- A template wizard for the cocreation of machine-readable data-reporting to harmonize the evaluation of (nano)materials.Nature protocols · 2024Review
- Air Pollution and Primary DNA Damage among Zagreb (Croatia) Residents: A Cross-Sectional Study.Journal of xenobiotics · 2024Article
- Optimizing the Comet Assay-Based In Vitro DNA Repair Assay for Placental Tissue: A Pilot Study with Pre-Eclamptic Patients.International journal of molecular sciences · 2023Article
- Entinostat Enhances the Efficacy of Chemotherapy in Small Cell Lung Cancer Through S-phase Arrest and Decreased Base Excision Repair.Clinical cancer research : an official journal of the American Association for Cancer Research · 2023Article
- Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Hedyotis diffusae Herba-Andrographis Herba inhibits the cellular proliferation of nasopharyngeal carcinoma and triggers DNA damage through activation of p53 and p21.Cancer gene therapy · 2022Article
- Quantifying DNA damage on paper sensorsChemical science · 2022Article
- Cytotoxicity of Metal-Based Nanoparticles: From Mechanisms and Methods of Evaluation to Pathological Manifestations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Cell survival after DNA damage in the comet assay.Archives of toxicology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This optimized protocol (including links to instruction videos) describes a comet-based in vitro DNA repair assay that is relatively simple, versatile, and inexpensive, enabling the detection of base and nucleotide excision repair activity. Protein extracts from samples are incubated with agarose-embedded substrate nucleoids ('naked' supercoiled DNA) containing specifically induced DNA lesions (e.g., resulting from oxidation, UVC radiation or benzo[a]pyrene-diol epoxide treatment). DNA incisions produced during the incubation reaction are quantified as strand breaks after electrophoresis, reflecting the extract's incision activity. The method has been applied in cell culture model systems, human biomonitoring and clinical investigations, and animal studies, using isolated blood cells and various solid tissues. Once extracts and substrates are prepared, the assay can be completed within 2 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.