ArticleNature communications2020
A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 104 citations in OpenAlex.
- Harnessing two UDP-glycosyltransferases from Saponaria officinalis toward heterologous production of endosomal escape enhancer saponins in Gypsophila elegans hairy roots.The Plant journal : for cell and molecular biology · 2026Article
- T2T and chromosome-level genome assemblies provide insights into the genetic basis of bioactive compound biosynthesis and environmental adaptation in licorice.Science China. Life sciences · 2026Article
- Complete biosynthesis of the clinical agent escin Ia.Nature communications · 2026Article
- Advances in UDP-Glycosyltransferases from Medicinal Plants: Discovery, Catalytic Mechanism, Engineering and Biosynthetic Application.Metabolites · 2026Review
- Gap Analysis of Metabolic Conversions of Off-Flavors and Antinutrients in Plant-Based Substrates.Comprehensive reviews in food science and food safety · 2026Review
- Review
- Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa.Plant biotechnology journal · 2025Article
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- Overwhelming glycyrrhizin production in Glycyrrhiza glabra induced by rihizobial symbiosis.Journal of natural medicines · 2025Article
- Article
- Unlocking saponin biosynthesis in soapwort.Nature chemical biology · 2025Article
- In planta ectopic expression of two subtypes of tomato cellulose synthase-like M genes affects cell wall integrity and supports a role in arabinogalactan and/or rhamnogalacturonan-I biosynthesis.Plant & cell physiology · 2025Article
- Simultaneous site-directed mutagenesis for soybean ß-amyrin synthase genes via DNA-free CRISPR/Cas9 system using a single gRNA.Plant cell reports · 2025Article
- Estimation of the heritability of medicinal compound contents in Glycyrrhiza uralensis.PloS one · 2025Article
- Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins.Frontiers in plant science · 2025Review
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- Triterpene RDF: Developing a database of plant enzymes and transcription factors involved in triterpene biosynthesis using the Resource Description Framework.Plant biotechnology (Tokyo, Japan) · 2024Article
- Application of Monoclonal Antibodies against Naturally Occurring Bioactive Ingredients.Antibodies (Basel, Switzerland) · 2024Review
- Advances in Engineering Nucleotide Sugar Metabolism for Natural Product Glycosylation inACS synthetic biology · 2024Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triterpenoid saponins are specialised metabolites distributed widely in the plant kingdom that consist of one or more sugar moieties attached to triterpenoid aglycones. Despite the widely accepted view that glycosylation is catalysed by UDP-dependent glycosyltransferase (UGT), the UGT which catalyses the transfer of the conserved glucuronic acid moiety at the C-3 position of glycyrrhizin and various soyasaponins has not been determined. Here, we report that a cellulose synthase superfamily-derived glycosyltransferase (CSyGT) catalyses 3-O-glucuronosylation of triterpenoid aglycones. Gene co-expression analyses of three legume species (Glycyrrhiza uralensis, Glycine max, and Lotus japonicus) reveal the involvement of CSyGTs in saponin biosynthesis, and we characterise CSyGTs in vivo using Saccharomyces cerevisiae. CSyGT mutants of L. japonicus do not accumulate soyasaponin, but the ectopic expression of endoplasmic reticulum membrane-localised CSyGTs in a L. japonicus mutant background successfully complement soyasaponin biosynthesis. Finally, we produced glycyrrhizin de novo in yeast, paving the way for sustainable production of high-value saponins.
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