Evidence map›Paper›PMID 33195515›Full record

ArticleFrontiers in veterinary science2020

Field-Adapted Full Genome Sequencing of Peste-Des-Petits-Ruminants Virus Using Nanopore Sequencing.

Emeli Torsson, Tebogo Kgotlele, Gerald Misinzo, Jonas Johansson Wensman, Mikael Berg, Oskar Karlsson Lindsjö

Open access · goldAbstract read
In one paragraph

Article in Frontiers in veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Emeli TorssonDepartment of Biomedical Sciences & Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Tebogo KgotleleDepartment of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro, Tanzania.
Gerald MisinzoDepartment of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro, Tanzania.
Jonas Johansson WensmanDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Mikael BergDepartment of Biomedical Sciences & Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Oskar Karlsson LindsjöDepartment of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Swedish University of Agricultural Sciences · SESokoine University of Agriculture · TZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peste-des-petits-ruminants virus (PPRV) is currently the focus of a control and eradication program. Full genome sequencing has the opportunity to become a powerful tool in the eradication program by improving molecular epidemiology and the study of viral evolution. PPRV is prevalent in many resource-constrained areas, with long distances to laboratory facilities, which can lack the correct equipment for high-throughput sequencing. Here we present a protocol for near full or full genome sequencing of PPRV. The use of a portable miniPCR and MinION brings the laboratory to the field and in addition makes the production of a full genome possible within 24 h of sampling. The protocol has been successfully used on virus isolates from cell cultures and field isolates from tissue samples of naturally infected goats.

Indexed as

eradicationfull genome sequencingMinIONminiPCRmolecular epidemiologypeste-des-petits-ruminants virus

Identifiers

PMID33195515
PMCPMC7649132
OpenAlexW3095725927

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.