Evidence map›Paper›PMID 33194417›Full record

ArticlePeerJ2020

Comparing sediment DNA extraction methods for assessing organic enrichment associated with marine aquaculture.

John K Pearman, Nigel B Keeley, Susanna A Wood, Olivier Laroche, Anastasija Zaiko, Georgia Thomson-Laing, Laura Biessy, Javier Atalah, Xavier Pochon

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 62 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Comparative analysis of endophyte diversity ofPlant biotechnology (Tokyo, Japan) · 2023
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

John K PearmanCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.
Nigel B KeeleyInstitute of Marine Research in Norway, Tromsø, Norway.ORCID 0000-0002-3397-8457
Susanna A WoodCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.ORCID 0000-0003-1976-8266
Olivier LarocheInstitute of Marine Research in Norway, Tromsø, Norway.ORCID 0000-0003-0755-0083
Anastasija ZaikoCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.
Georgia Thomson-LaingCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.ORCID 0000-0001-8337-5489
Laura BiessyCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.ORCID 0000-0001-8749-1495
Javier AtalahCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.ORCID 0000-0003-0625-3431
Xavier PochonCoastal and Freshwater Group, Cawthron Institute, Nelson, New Zealand.
Cawthron Institute · NZNorwegian Institute of Marine Research · NOUniversity of Auckland · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marine sediments contain a high diversity of micro- and macro-organisms which are important in the functioning of biogeochemical cycles. Traditionally, anthropogenic perturbation has been investigated by identifying macro-organism responses along gradients. Environmental DNA (eDNA) analyses have recently been advocated as a rapid and cost-effective approach to measuring ecological impacts and efforts are underway to incorporate eDNA tools into monitoring. Before these methods can replace or complement existing methods, robustness and repeatability of each analytical step has to be demonstrated. One area that requires further investigation is the selection of sediment DNA extraction method. Environmental DNA sediment samples were obtained along a disturbance gradient adjacent to a Chinook (

Indexed as

16S rRNA gene18S rRNA geneBenthic monitoringDNA extractionEnvironmental DNAMetabarcoding

Identifiers

PMID33194417
PMCPMC7597629
OpenAlexW3094776990

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.