Evidence map›Paper›PMID 33193274›Full record

ReviewFrontiers in microbiology2020

Bacteriophage Enumeration and Detection Methods.

Norbert Ács, Michela Gambino, Lone Brøndsted

Abstract readReview
In one paragraph

Review in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Robust detection in faeces andVeterinary and animal science · 2026
    Article
  4. Article
  5. Article
  6. Isolation and characterization of broad-rangeMicrobiology (Reading, England) · 2026
    Article
  7. Article
  8. Article
  9. Isolation, characterisation, and biocontrol application of novel Clostridium perfringens phages on chicken meat.International microbiology : the official journal of the Spanish Society for Microbiology · 2026
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Newly isolatedFrontiers in microbiology · 2026
    Article
  15. Genomic and functional characterization ofFrontiers in microbiology · 2026
    Article
  16. Review
  17. Article
  18. Article
  19. Review
  20. Article

11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Norbert ÁcsDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Michela GambinoDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Lone BrøndstedDepartment of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Application of phages as alternative antimicrobials to combat pathogenic bacteria and their association to a healthy gut microbiome has prompted a need for precise methods for detection and enumeration of phage particles. There are many applicable methods, but care should be taken considering the measured object (infectious phage, whole phage particle or nucleic acid and proteins) and the concept behind the technique to avoid misinterpretations. While molecular methods cannot discriminate between viable and non-infectious phages, the traditional techniques for counting infectious phages can be time consuming and poorly reproducible. Here, we describe the methods currently used for phage detection and enumeration and highlight their advantages as well as their limitations. Finally, we provide insight on how to deal with complex samples, as well as future prospects in the field of phage quantification.

Indexed as

bacteriophagedetectiondouble agar overlay assayenumerationmolecular biologyreal-time PCRsequencing

Identifiers

PMID33193274
PMCPMC7644846

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.