ArticleFrontiers in cellular neuroscience2020
Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical Neurons.
Article in Frontiers in cellular neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 29 citations in OpenAlex.
- Neocortical pyramidal cells with axon-carrying basal dendrites: a prominent feature regulated by activity.Brain structure & function · 2026Article
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- Transcranial Calcium Macro-Imaging From the Auditory Cortex of Thy1-Cre-Driven GCaMP8 Transgenic Rats.Neuropsychopharmacology reports · 2026Article
- Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpoles.STAR protocols · 2026Article
- Perinatal mAChR-mediated activation of cortical subplate neurons elicits network activity driving basket cell differentiation.Frontiers in cellular neuroscience · 2026Article
- The Multifaceted Role of Calcium Signaling in Regulated Necrosis.Biomolecules · 2025Review
- Two-point calibration protocol for the Förster Resonance Energy Transfer indicator Pyronic in neurons.Neurophotonics · 2025Article
- Defective Mammary Epithelial Outgrowth in Transgenic EKAREV-NLS Mice: Correction via Estrogen Supplementation and Genetic Background Modification.Journal of mammary gland biology and neoplasia · 2025Article
- Chemogenetic activation of Gq signaling modulates dendritic development of cortical neurons in a time- and layer-specific manner.Frontiers in cellular neuroscience · 2025Article
- Aberrant hippocampal CaeLife · 2024Article
- Wide-field imaging in behaving mice as a tool to study cognitive function.Neurophotonics · 2024Review
- Aberrant hippocampal CabioRxiv : the preprint server for biology · 2024Article
- Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.Frontiers in neuroscience · 2024Review
- Investigating the effect of Shenmai injection on cardiac electrophysiology and calcium signaling using human-induced pluripotent stem cell-derived cardiomyocytes.Biochemistry and biophysics reports · 2023Article
- Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo.Journal of neurochemistry · 2023Review
- Optogenetic stimulation shapes dendritic trees of infragranular cortical pyramidal cells.Frontiers in cellular neuroscience · 2023Article
- The GEM-GECO Calcium Indicator Is Useable inInternational journal of molecular sciences · 2022Article
- Encoding, transmission, decoding, and specificity of calcium signals in plants.Journal of experimental botany · 2022Review
- Letting the little light of mind shine: Advances and future directions in neurochemical detection.Neuroscience research · 2022Review
- Chemogenetic Silencing of Differentiating Cortical Neurons Impairs Dendritic and Axonal Growth.Frontiers in cellular neuroscience · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A battery of genetically encoded calcium indicators (GECIs) with different binding kinetics and calcium affinities was developed over the recent years to permit long-term calcium imaging. GECIs are calcium buffers and therefore, expression of GECIs may interfere with calcium homeostasis and signaling pathways important for neuronal differentiation and survival. Our objective was to investigate if the biolistically induced expression of five commonly used GECIs at two postnatal time points (days 14 and 22-25) could affect the morphological maturation of cortical neurons in organotypic slice cultures of rat visual cortex. Expression of GCaMP3 in both time windows, and of GCaMP5G and TN-XXL in the later time window impaired apical and /or basal dendrite growth of pyramidal neurons. With time, the proportion of GECI transfectants with nuclear filling increased, but an only prolonged expression of TN-XXL caused higher levels of neurodegeneration. In multipolar interneurons, only GCaMP3 evoked a transient growth delay during the early time window. GCaMP6m and GCaMP6m-X
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.