ArticleJournal of visualized experiments : JoVE2020
An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 7 citations in OpenAlex.
- A Multifunctional Shape-Adaptive Bilayer Hydrogel for Acute Hemostasis, Wound Repair, and Insect Bite Defense.Gels (Basel, Switzerland) · 2026Article
- Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions iniScience · 2026Article
- Towards Bioethical and Functional Standards in the Slaughter Methods of Edible Insects: A Narrative Review.Insects · 2026Review
- ILP4 and InR regulate paclitaxel-induced hypersensitivity differently inMolecular biology of the cell · 2026Article
- Mechanical Nociception Assay inCold Spring Harbor protocols · 2025Article
- The insulin receptor regulates the persistence of mechanical nociceptive sensitization in flies and mice.Biology open · 2023Article
- Nociception in fruit fly larvae.Frontiers in pain research (Lausanne, Switzerland) · 2023Review
- Evaluating Baseline and Sensitised Heat Nociception in AdultBio-protocol · 2021Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Published assays for mechanical nociception in Drosophila have led to variable assessments of behavior. Here, we fabricated, for use with Drosophila larvae, customized metal nickel-titanium alloy (nitinol) filaments. These mechanical probes are similar to the von Frey filaments used in vertebrates to measure mechanical nociception. Here, we demonstrate how to make and calibrate these mechanical probes and how to generate a full behavioral dose-response from subthreshold (innocuous or non-noxious range) to suprathreshold (low to high noxious range) stimuli. To demonstrate the utility of the probes, we investigated tissue damage-induced hypersensitivity in Drosophila larvae. Mechanical allodynia (hypersensitivity to a normally innocuous mechanical stimulus) and hyperalgesia (exaggerated responsiveness to a noxious mechanical stimulus) have not yet been established in Drosophila larvae. Using mechanical probes that are normally innocuous or probes that typically elicit an aversive behavior, we found that Drosophila larvae develop mechanical hypersensitization (both allodynia and hyperalgesia) after tissue damage. Thus, the mechanical probes and assay that we illustrate here will likely be important tools to dissect the fundamental molecular/genetic mechanisms of mechanical hypersensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.