Evidence map›Paper›PMID 33190422›Full record

ArticleFEBS open bio2021

Isolation and thermal stabilization of mouse ferroportin.

Chandrika N Deshpande, Corbin R Azucenas, Bo Qiao, Norimichi Nomura, Vicky Xin, Josep Font, So Iwata, Tomas Ganz, Elizabeta Nemeth, Bryan Mackenzie and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 4 countries.

Chandrika N DeshpandeStructural Biology Program, Centenary Institute, Sydney, NSW, Australia.
Corbin R AzucenasDepartment of Pharmacology & Systems Physiology, University of Cincinnati College of Medicine, OH.ORCID 0000-0003-1674-2407
Bo QiaoDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Norimichi NomuraDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Japan.
Vicky XinStructural Biology Program, Centenary Institute, Sydney, NSW, Australia.
Josep FontStructural Biology Program, Centenary Institute, Sydney, NSW, Australia.
So IwataDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Japan.
Tomas GanzDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.ORCID 0000-0002-2830-5469
Elizabeta NemethDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.ORCID 0000-0002-3477-2397
Bryan MackenzieMedical Sciences Baccalaureate Program, University of Cincinnati College of Medicine, OH, USA.ORCID 0000-0002-9661-8838
Mika JormakkaStructural Biology Program, Centenary Institute, Sydney, NSW, Australia.ORCID 0000-0002-2326-1735
Centenary Institute · AUUniversity of California, Los Angeles · USInternational Baccalaureate · CHKyoto University · JP

Funding

Stem Cell/Organoid and Genome Editing CoreP30DK078392 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI Alexander Miethke · 2007 to 2026
$24.4M
Ferroportin Structure and functionR01DK107309 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JORMAKKA, MIKA, MACKENZIE, BRYAN · 2015 to 2019
$3.0M
The Hepcidin-Ferroportin Axis in Anemia of Inflammation: Mechanisms and TargetsR01DK082717 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI NEMETH, ELIZABETA · 2008 to 2012
$1.6M
APS Short-Term Research Education Program to Increase Diversity in Health-RelatedR25HL115473 · NHLBI · AMERICAN PHYSIOLOGICAL SOCIETY · PI BRUTHERS, CHERYL BROOKE, MATYAS, MARSHA LAKES · 2012 to 2017
$853k
NHLBI NIH HHS R25 HL115473NIDDK NIH HHS P30 DK078392NIDDK NIH HHS R01 DK082717NIDDK NIH HHS R01 DK107309
6 · The paper itself

Abstract

Ferroportin (Fpn) is an essential mammalian iron transporter that is negatively regulated by the hormone hepcidin. Our current molecular understanding of Fpn-mediated iron efflux and regulation is limited due to a lack of biochemical, biophysical and high-resolution structural studies. A critical step towards understanding the transport mechanism of Fpn is to obtain sufficient quantities of pure and stable protein for downstream studies. As such, we detail here an expression and purification protocol for mouse Fpn yielding milligram quantities of pure protein. We have generated deletion constructs exhibiting enhanced thermal stability and which retained iron-transport activity and hepcidin responsiveness, providing a platform for further biophysical studies of Fpn.

Indexed as

AnimalsCation Transport ProteinsFerroportinHepcidinsHot TemperatureMiceProtein StabilityRecombinant ProteinsCation Transport ProteinsFerroportinHepcidinsRecombinant Proteinsferroportinhepcidin regulationiron metabolismthermostabilization

Identifiers

PMID33190422
PMCPMC7780100
OpenAlexW3098101273

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.