ArticleBiophysical journal2020
Differential Diffusional Properties in Loose and Tight Docking Prior to Membrane Fusion.
Article in Biophysical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed.
- Number and relative abundance of synaptic vesicles in functionally distinct priming states determine synaptic strength and short-term plasticity.The Journal of physiology · 2025Article
- Multiparametric functional characterization of individual lipid nanoparticles using surface-sensitive light-scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Observing isolated synaptic vesicle association and fusion ex vivo.Nature protocols · 2024Review
- Intermediate steps in the formation of neuronal SNARE complexes.The Journal of biological chemistry · 2024Article
- Fully-primed slowly-recovering vesicles mediate presynaptic LTP at neocortical neurons.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Forty years of the adrenal chromaffin cell through ISCCB meetings around the world.Pflugers Archiv : European journal of physiology · 2023Review
- Tight docking of membranes before fusion represents a metastable state with unique properties.Nature communications · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fusion of biological membranes, although mediated by divergent proteins, is believed to follow a common pathway. It proceeds through distinct steps, including docking, merger of proximal leaflets (stalk formation), and formation of a fusion pore. However, the structure of these intermediates is difficult to study because of their short lifetime. Previously, we observed a loosely and tightly docked state preceding leaflet merger using arresting point mutations in SNARE proteins, but the nature of these states remained elusive. Here, we used interferometric scattering (iSCAT) microscopy to monitor diffusion of single vesicles across the surface of giant unilamellar vesicles (GUVs). We observed that the diffusion coefficients of arrested vesicles decreased during progression through the intermediate states. Modeling allowed for predicting the number of tethering SNARE complexes upon loose docking and the size of the interacting membrane patches upon tight docking. These results shed new light on the nature of membrane-membrane interactions immediately before fusion.
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Registered trials
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