ArticleInsect biochemistry and molecular biology2020
Heterogeneity of midgut cells and their differential responses to blood meal ingestion by the mosquito, Aedes aegypti.
Article in Insect biochemistry and molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 74 citations in OpenAlex.
- Single-cell RNA sequencing unravels mosquito biology and host-pathogen interactions.Parasites & vectors · 2026Review
- Vector competence acrossJournal of virology · 2026Review
- Parallel expansion of trehalose transporter genes integrates intestinal energy metabolism with developmental growth in lepidopteran insects.Molecular biology and evolution · 2026Article
- Population bottlenecks and temporal constraints shape dengue virus infection of Aedes aegypti midguts.PLoS neglected tropical diseases · 2026Article
- Optimized Method for High-Quality Isolation of Single-Nuclei From Mosquito Fat Body for RNA Sequencing.Bio-protocol · 2026Article
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- MappingbioRxiv : the preprint server for biology · 2025Article
- Cellular and molecular keys to entry: Mechanisms mediating Orthoflavivirus infection of the mosquito midgut.PLoS pathogens · 2025Article
- A single-nucleus transcriptomic atlas of the adultbioRxiv : the preprint server for biology · 2025Article
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- TNF signaling mediates cellular immune function and promotes malaria parasite killing in the mosquito Anopheles gambiae.PLoS pathogens · 2025Article
- Biochemical and physiological characterization of Aedes aegypti midgut chymotrypsin.Scientific reports · 2025Article
- A comprehensive cell atlas of fall armyworm (Spodoptera frugiperda) larval gut and fat body via snRNA-Seq.Scientific data · 2025Article
- Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia.Scientific reports · 2025Article
- A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection.PLoS pathogens · 2025Article
- A single-cell atlas of thebioRxiv : the preprint server for biology · 2024Article
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- Reciprocal interactions between neuropeptide F and RYamide regulate host attraction in the mosquitoProceedings of the National Academy of Sciences of the United States of America · 2024Article
- A cell atlas of the adult female Aedes aegypti midgut revealed by single-cell RNA sequencing.Scientific data · 2024Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Mosquitoes are the most notorious hematophagous insects and due to their blood feeding behavior and genetic compatibility, numerous mosquito species are highly efficient vectors for certain human pathogenic parasites and viruses. The mosquito midgut is the principal organ of blood meal digestion and nutrient absorption. It is also the initial site of infection with blood meal acquired parasites and viruses. We conducted an analysis based on single-nucleus RNA sequencing (snRNA-Seq) to assess the cellular diversity of the midgut and how individual cells respond to blood meal ingestion to facilitate its digestion. Our study revealed the presence of 20 distinguishable cell-type clusters in the female midgut of Aedes aegypti. The identified cell types included intestinal stem cells (ISC), enteroblasts (EB), differentiating EB (dEB), enteroendocrine cells (EE), enterocytes (EC), EC-like cells, cardia cells, and visceral muscle (VM) cells. Blood meal ingestion dramatically changed the overall midgut cell type composition, profoundly increasing the proportions of ISC and three EC/EC-like clusters. In addition, transcriptional profiles of all cell types were strongly affected while genes involved in various metabolic processes were significantly upregulated. Our study provides a basis for further physiological and molecular studies on blood digestion, nutrient absorption, and cellular homeostasis in the mosquito midgut.
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