ArticleAngewandte Chemie (International ed. in English)2021
Spatiotemporal Measurement of Osmotic Pressures by FRET Imaging.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 12 citations in OpenAlex.
- Submicron-Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology.Advanced healthcare materials · 2023Article
- Spatiotemporal Measurement of Osmotic Pressures by FRET Imaging.Angewandte Chemie (International ed. in English) · 2021Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
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Abstract
Osmotic pressures (OPs) play essential roles in biological processes and numerous technological applications. However, the measurement of OP in situ with spatiotemporal resolution has not been achieved so far. Herein, we introduce a novel kind of OP sensor based on liposomes loaded with water-soluble fluorescent dyes exhibiting resonance energy transfer (FRET). The liposomes experience volume changes in response to OP due to water outflux. The FRET efficiency depends on the average distance between the entrapped dyes and thus provides a direct measure of the OP surrounding each liposome. The sensors exhibit high sensitivity to OP in the biologically relevant range of 0-0.3 MPa in aqueous solutions of salt, small organic molecules, and macromolecules. With the help of FRET microscopy, we demonstrate the feasibility of spatiotemporal OP imaging, which can be a promising new tool to investigate phenomena involving OPs and their dynamics in biology and technology.
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