Evidence map›Paper›PMID 33186380›Full record

ArticlePloS one2020

Tuning cell behavior with nanoparticle shape.

Edoardo Scarpa, Cesare De Pace, Adrian Steve Joseph, Senio Campos de Souza, Alessandro Poma, Eva Liatsi-Douvitsa, Claudia Contini, Valeria De Matteis, Josep Samitier Martí, Giuseppe Battaglia and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Edoardo ScarpaDepartment of Chemistry, University College London, London, United Kingdom.
Cesare De PaceDepartment of Chemistry, University College London, London, United Kingdom.
Adrian Steve JosephDepartment of Chemistry, University College London, London, United Kingdom.
Senio Campos de SouzaDepartment of Chemistry, University College London, London, United Kingdom.
Alessandro PomaDepartment of Chemistry, University College London, London, United Kingdom.
Eva Liatsi-DouvitsaDepartment of Chemistry, University College London, London, United Kingdom.
Claudia ContiniDepartment of Chemistry, University College London, London, United Kingdom.ORCID 0000-0002-5282-2458
Valeria De MatteisDepartment of Mathematics and Physics, University of Salento, Lecce, Italy.
Josep Samitier MartíInstitute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Barcelona, Spain.
Giuseppe BattagliaDepartment of Chemistry, University College London, London, United Kingdom.
Loris RizzelloDepartment of Chemistry, University College London, London, United Kingdom.ORCID 0000-0002-8230-853X
University College London · GBInstitució Catalana de Recerca i Estudis Avançats · ESUniversity of Milan · ITUniversity of Salento · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigated how the shape of polymeric vesicles, made by the exact same material, impacts the replication activity and metabolic state of both cancer and non-cancer cell types. First, we isolated discrete geometrical structures (spheres and tubes) from a heterogeneous sample using density-gradient centrifugation. Then, we characterized the cellular internalization and the kinetics of uptake of both types of polymersomes in different cell types (either cancer or non-cancer cells). We also investigated the cellular metabolic response as a function of the shape of the structures internalized and discovered that tubular vesicles induce a significant decrease in the replication activity of cancer cells compared to spherical vesicles. We related this effect to the significant up-regulation of the tumor suppressor genes p21 and p53 with a concomitant activation of caspase 3/7. Finally, we demonstrated that combining the intrinsic shape-dependent effects of tubes with the delivery of doxorubicin significantly increases the cytotoxicity of the system. Our results illustrate how the geometrical conformation of nanoparticles could impact cell behavior and how this could be tuned to create novel drug delivery systems tailored to specific biomedical application.

Indexed as

Caspase 3Caspase 7Cell Line, TumorCentrifugation, Density GradientCyclin-Dependent Kinase Inhibitor p21DNA ReplicationDoxorubicinHeLa CellsHumansNanoparticlesNeoplasmsTumor Suppressor Protein p53Up-RegulationCASP3 protein, humanCASP7 protein, humanCaspase 3Caspase 7CDKN1A protein, humanCyclin-Dependent Kinase Inhibitor p21DoxorubicinTP53 protein, humanTumor Suppressor Protein p53

Identifiers

PMID33186380
PMCPMC7665645
OpenAlexW3100211612

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.