Evidence map›Paper›PMID 33183278›Full record

ArticleBMC complementary medicine and therapies2020

Bark extract of Cassia sieberiana DC. (Caesalpiniaceae) displayed good antibacterial activity against MDR gram-negative phenotypes in the presence of phenylalanine-arginine β-naphthylamide.

Marilene M M Ambadiang, Brice C K Atontsa, Simplice B Tankeo, Paul Nayim, Brice E N Wamba, Gabin T M Bitchagno, James D S Mpetga, Veronique B Penlap, Victor Kuete

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary medicine and therapies, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Review
  3. Polyoxygenated Stigmastane-Type Steroids fromMolecules (Basel, Switzerland) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Marilene M M AmbadiangDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Brice C K AtontsaDepartment of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Simplice B TankeoDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Paul NayimDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Brice E N WambaDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Gabin T M BitchagnoDepartment of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
James D S MpetgaDepartment of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Veronique B PenlapDepartment of Biochemistry, University of Yaounde 1, P.O. Box 812, Cameroun, Yaounde, Cameroon.
Victor KueteDepartment of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon. kuetevictor@yahoo.fr.ORCID http://orcid.org/0000-0002-1070-1236
Université de Dschang · CMUniversité de Yaoundé I · CM

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMultidrug-resistant (MDR) bacteria remain a major cause of morbidity and mortality globally. The present study was designed to investigate the in vitro antibacterial activities of crude methanol extract and constituents isolated by Column Chromatography (CC) from Cassia sieberiana bark (CSB) against ten MDR Gram-negative bacteria, as well as the mechanisms of action of the most active sample.

methodsThe antibacterial activity of the tested samples (extract, the fractions and their compounds isolated by CC and the structures obtained by exploiting

resultsPhytochemical composition of the crude extract showed a rather selective distribution of secondary metabolites (presence of polyphenols, tannins, steroids, triterpenes, flavonoids, alkaloids, saponins and absence of anthocyanins, anthraquinones). The tested samples displayed different antibacterial activities with minimal inhibitory concentrations (MICs) ranging from 64 to 512 μg/mL. Crude extract (CS) and fraction CSc showed the highest inhibitory spectra, both inhibiting all of the studied bacteria except Enterobacter aerogenes EA27 strain. Fraction CSc exerted bactericidal effects on most bacteria meanwhile, crude extract (CS) and sub-fraction CSc2 exerted bacteriostatic effects. Compounds 1 (spectaline) and 2 (iso-6-cassine) inhibited the growth of 70% (Escherichia coli ATCC8739 and AG102, Klebsiella pneumoniae ATCC11296, Enterobacter aerogenes ATCC13048 and EA27, Providencia stuartii ATCC29916, Pseudomonas aeruginosa PA01) and 60% (Escherichia coli ATCC8739, Klebsiella pneumoniae ATCC11296 and KP55, Providencia stuartii ATCC29916, Pseudomonas aeruginosa PA01 and PA124) of bacteria respectively with MICs ranging from 128 to 512 μg/mL. In the presence of PAβN, the activities of crude extract CS, fraction CAc and sub-fraction CSc2 strongly increased on most bacteria strains as their MICs significantly decreased. Sub-fraction CSc2 inhibited the H

conclusionThe overall results justify the traditional use of C. sieberiana for the treatment of bacterial infections.

Indexed as

Anti-Bacterial AgentsBacterial ProteinsCassiaDipeptidesDrug Resistance, Multiple, BacterialGram-Negative BacteriaMicrobial Sensitivity TestsPlant BarkPlant ExtractsProton-Translocating ATPasesAnti-Bacterial AgentsBacterial ProteinsDipeptidesphenylalanine arginine beta-naphthylamidePlant ExtractsProton-Translocating ATPasesCassia sieberianaEfflux pumpsGram negative bacteriaInfectious diseasesMultidrug resistance

Identifiers

PMID33183278
PMCPMC7664092
OpenAlexW3103259883

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.