Evidence map›Paper›PMID 33183225›Full record

ArticleBMC evolutionary biology2020

DNA barcoding reveals cryptic diversity in the underestimated genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the northeastern Qinghai-Tibet Plateau.

Tai Wang, Yan-Ping Zhang, Zhuo-Yu Yang, Zhe Liu, Yan-Yan Du

Open access · goldAbstract read
In one paragraph

Article in BMC evolutionary biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 42 citations in OpenAlex.

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  15. Phylogeography ofGenes · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Tai WangCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Yan-Ping ZhangGansu Key Laboratory of Cold Water Fishes Germplasm Resources and Genetics Breeding, Gansu Fisheries Research Institute, Lanzhou, China.
Zhuo-Yu YangGansu Key Laboratory of Cold Water Fishes Germplasm Resources and Genetics Breeding, Gansu Fisheries Research Institute, Lanzhou, China.
Zhe LiuCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China. 5279292@qq.com.
Yan-Yan DuGansu Key Laboratory of Cold Water Fishes Germplasm Resources and Genetics Breeding, Gansu Fisheries Research Institute, Lanzhou, China. 273639273@qq.com.
Gansu Agricultural University · CN

Funding

National Natural Science Foundation of China 31460560
6 · The paper itself

Abstract

backgroundThe northeastern part of the Qinghai-Tibet Plateau (QTP) presents a high number of plateau loach species. As one of the three major groups of fishes distributed on the QTP, plateau loach has high ecological value. However, the taxonomy and systematics of these fish are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and many cryptic species are observed. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern part of the QTP and reveal the hidden diversity by comparing morphological species with molecular operational taxonomic units (MOTUs).

resultsAfter careful identification and comparison of the morphology and DNA barcoding of 1630 specimens, 22 species were identified, with 20 considered valid local species and two identified as new species that had not been previously described. Based on the combination of morphological and molecular methods, a total of 24 native species were found, two of which were cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes that allow them to be reliably identified. The remaining cases involved 10 closely related species, including rapidly differentiated species and species that seemed to have experienced incomplete lineage sorting or showed introgressions.

conclusionsThe results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially closely related species, such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.

Indexed as

CypriniformesDNA Barcoding, TaxonomicPhylogenyAnimalsDNATibetDNACryptic speciesDNA barcodeQinghai-TibetTibetan loach

Identifiers

PMID33183225
PMCPMC7663858
OpenAlexW3101077257

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.