ArticleCell stem cell2021
A Hierarchy of Proliferative and Migratory Keratinocytes Maintains the Tympanic Membrane.
Article in Cell stem cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Fibrous Biomaterial Scaffold for Tympanic Membrane Repair: Microarchitectural Engineering and Structure Function Performance.Journal of functional biomaterials · 2026Review
- Mapping the Regenerative Pattern in the Human Tympanic Membrane.Audiology & neuro-otology · 2026Article
- Postoperative Otorrhea as a Predictor of Early Ventilation Tube Extrusion in Children.Turkish archives of otorhinolaryngology · 2025Article
- Single cell transcriptomics of human psoriasis and epidermal specific Ube2l3 deficient mice highlight CXCL16/CXCR6 involvement in psoriasis development.Nature communications · 2025Article
- Tracking cell layer contribution during repair of the tympanic membrane.Disease models & mechanisms · 2024Article
- Comparison of healing of acute total tympanic membrane perforation between rats with and without excision of the mallear handle.Laryngoscope investigative otolaryngology · 2023Article
- Epimorphic regeneration in the mammalian tympanic membrane.NPJ Regenerative medicine · 2023Article
- Article
- Inhibition of TGF-β signaling enables long-term proliferation of mouse primary epithelial stem/progenitor cells of the tympanic membrane and the middle ear mucosa.Scientific reports · 2023Article
- Thymosin beta-4 - A potential tool in healing middle ear lesions in adult mammals.International immunopharmacology · 2023Article
- Developmental aspects of the tympanic membrane: Shedding light on function and disease.Genesis (New York, N.Y. : 2000) · 2020Review
- Anatomy and Development of the Mammalian External Auditory Canal: Implications for Understanding Canal Disease and Deformity.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The tympanic membrane (TM) is critical for hearing and requires continuous clearing of cellular debris, but little is known about homeostatic mechanisms in the TM epidermis. Using single-cell RNA sequencing, lineage tracing, whole-organ explant, and live-cell imaging, we show that homeostatic TM epidermis is distinct from other epidermal sites and has discrete proliferative zones with a three-dimensional hierarchy of multiple keratinocyte populations. TM stem cells reside in a discrete location of the superior TM and generate long-lived clones and committed progenitors (CPs). CP clones exhibit lateral migration, and their proliferative capacity is supported by Pdgfra+ fibroblasts, generating migratory but non-proliferative progeny. Single-cell sequencing of the human TM revealed similar cell types and transcriptional programming. Thus, during homeostasis, TM keratinocytes transit through a proliferative CP state and exhibit directional lateral migration. This work forms a foundation for understanding TM disorders and modeling keratinocyte biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.