ArticleeLife2020
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.
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Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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106citing papers in PubMed
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Who cites it
106 citing papers in PubMed.
- A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging.bioRxiv : the preprint server for biology · 2026Article
- Arachidonic acid availability controls neutrophil swarm initiation and scaling.bioRxiv : the preprint server for biology · 2026Article
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity.Scientific data · 2026Article
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- Article
- Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.Biomedical optics express · 2026Article
- Versatile high-speed volumetric imaging from microscopic to macroscopic scale by self-adaptive oblique plane microscopy.bioRxiv : the preprint server for biology · 2026Article
- Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Rapid and gentle volumetric imaging of host-pathogen interactions in salmon skin cells using projective oblique plane microscopy.Scientific reports · 2025Article
- High content 3D imaging by dual-view oblique plane microscopy.PNAS nexus · 2025Article
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet Fluorescence Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Ultrastructural membrane dynamics of mouse and human cortical synapses.bioRxiv : the preprint server for biology · 2025Article
- Cleared tissue dual-view oblique plane microscopy.Biomedical optics express · 2025Article
- Adaptive-learning physics-assisted light-field microscopy enables day-long and millisecond-scale super-resolution imaging of 3D subcellular dynamics.Nature communications · 2025Article
- Tuning collagen nonlinear mechanics with interpenetrating networks drives adaptive cellular phenotypes in three dimensions.Science advances · 2025Article
- Ultrafast endocytosis in mouse cortical inhibitory synapses.bioRxiv : the preprint server for biology · 2025Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
25 authors.
Etai SapoznikDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0001-8472-0299
Bo-Jui ChangDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0002-5513-7106
Jaewon HuhDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0003-3954-8092
Robert J JuInstitute for Molecular Bioscience, University of Queensland, Queensland, Australia.ORCID https://orcid.org/0000-0002-9850-9803
Evgenia V AzarovaDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0002-3846-9176
Theresa PohlkampDepartment of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, United States.ORCID http://orcid.org/0000-0003-3923-1917
Erik S WelfDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.
David BroadbentInstitute for Quantitative Health Sciences and Engineering, Michigan State University, East Lansing, United States.ORCID https://orcid.org/0000-0002-0940-1068
Alexandre F CariseyWilliam T. Shearer Center for Human Immunobiology, Baylor College of Medicine and Texas Children's Hospital, Houston, United States.ORCID http://orcid.org/0000-0003-1326-2205
Samantha J StehbensInstitute for Molecular Bioscience, University of Queensland, Queensland, Australia.ORCID https://orcid.org/0000-0002-8145-2708
Kyung-Min LeeHarold C. Simmons Comprehensive Cancer Center and the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States.
Arnaldo MarínHarold C. Simmons Comprehensive Cancer Center and the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States.
Ariella B HankerHarold C. Simmons Comprehensive Cancer Center and the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0002-8655-8341
Jens C SchmidtInstitute for Quantitative Health Sciences and Engineering, Michigan State University, East Lansing, United States.ORCID http://orcid.org/0000-0001-9061-7853
Carlos L ArteagaHarold C. Simmons Comprehensive Cancer Center and the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States.
Bin YangChan Zuckerberg Biohub, San Francisco, United States.
Yoshihiko KobayashiDepartment of Cell Biology, Duke University School of Medicine, Durham, United States.ORCID http://orcid.org/0000-0001-7031-1478
Purushothama Rao TataDepartment of Cell Biology, Duke University School of Medicine, Durham, United States.ORCID http://orcid.org/0000-0003-4837-0337
Rory KruithoffCenter for Biological Physics and Department of Physics, Arizona State University, Tempe, United States.
Konstantin DoubrovinskiDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.
Douglas P ShepherdCenter for Biological Physics and Department of Physics, Arizona State University, Tempe, United States.ORCID https://orcid.org/0000-0001-9087-0832
Alfred Millett-SikkingCalico Life Sciences LLC, South San Francisco, United States.
Andrew G YorkCalico Life Sciences LLC, South San Francisco, United States.
Kevin M DeanDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0003-0839-2320
Reto P FiolkaDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0002-4636-5000
Funding
UT Southwestern Medical Center Simmons Comprehensive Cancer CenterP30CA142543 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Sandi Leigh Pruitt · 2010 to 2026
$53.7MVanderbilt Clinical Oncology Research Career Development ProgramK12CA090625 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Debra L. Friedman, Paula Jill Hurley · 2001 to 2026
$16.9MRole of VEGF in Perinatal Pulmonary HypertensionR01HL068702 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI ABMAN, STEVEN HERBERT, SHEPHERD, DOUGLAS · 2001 to 2021
$6.7MTransformative microscopes to image across spatiotemporal scalesR35GM133522 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Reto Paul Fiolka · 2019 to 2026
$3.1MPrune Belly Syndrome: Mechanisms of Filamin A MutationsR01DK127589 · NIDDK · UT SOUTHWESTERN MEDICAL CENTER · PI BAKER, LINDA A., QIN, JUN · 2020 to 2024
$2.5MNeuronal signaling mechanisms of stress-induced anhedonia in the lateral habenulaR01MH120131 · NIMH · UT SOUTHWESTERN MEDICAL CENTER · PI SHABEL, STEVEN · 2020 to 2024
$2.4MRole of HER2 mutations in breast cancer progression and response to targeted therapiesR01CA224899 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI ARTEAGA, CARLOS L · 2018 to 2022
$1.8MMultiscale microscope for 3D cancer imaging in model organisms and organoidsR33CA235254 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI FIOLKA, RETO PAUL · 2019 to 2021
$930kMolecular Mechanisms of Telomerase Catalysis and its Recruitment to TelomeresR00GM120386 · NIGMS · MICHIGAN STATE UNIVERSITY · PI SCHMIDT, JENS CHRISTOPHER · 2018 to 2020
$747kLinking function, structure, and molecular identity of lateral habenula neuronsR34NS121873 · NINDS · UT SOUTHWESTERN MEDICAL CENTER · PI SHABEL, STEVEN · 2021 to 2021
$738kMathematical models for rotary protein motors driven by membrane potentialR01GM110066 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MANDADAPU, KRANTHI KIRAN · 2015 to 2017
$634kMechanisms of melanoma migration and survival in 3D microenvironmentsK25CA204526 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI WELF, ERIK S · 2016 to 2020
$585kAmes Research Center NASA FT190100516Damon Runyon Cancer Research Foundation DFS-24-17NCI NIH HHS K12 CA090625NCI NIH HHS K25 CA204526NCI NIH HHS P30 CA142543NCI NIH HHS R01 CA224899NCI NIH HHS R33 CA235254NHLBI NIH HHS R01 HL068702NIDDK NIH HHS R01 DK127589NIGMS NIH HHS R00 GM120386NIGMS NIH HHS R01 GM110066NIGMS NIH HHS R35 GM133522NIH HHS 1R01MH120131-01A1NIH HHS 1R34NS121873NIH HHS K25 CA204526NIH HHS P30 CA142543NIH HHS R00 GM120386NIH HHS R01GM110066NIH HHS R01HL068702NIMH NIH HHS R01 MH120131NINDS NIH HHS R34 NS121873
Abstract
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.
Indexed as
AnimalsCells, CulturedHumansMiceMicrofluidic Analytical TechniquesMicroscopy, ConfocalPlasmidsRatsSingle-Cell Analysiscell biologyfluorescencelight-sheetmicrofluidicsmicroscopynoneoptogeneticsparticle tracking
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.