ArticlePlant biotechnology journal2021
Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Synthetic transcriptional repression systems in plants.Plant cell reports · 2026Review
- Robust quantification of multiplexed fluorescent protein-based biosensors in plant tissues.The Plant journal : for cell and molecular biology · 2026Article
- Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria.Nature biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Simulations predict stronger CRISPRi transcriptional repression in plants for identical than heterogeneous gRNA target sites.Synthetic biology (Oxford, England) · 2025Article
- Drought stress-inducible synthetic promoters designed for poplar are functional in rice.Plant cell reports · 2024Article
- Engineered gamma radiation phytosensors for environmental monitoring.Plant biotechnology journal · 2023Article
- Designing artificial synthetic promoters for accurate, smart, and versatile gene expression in plants.Plant communications · 2023Review
- Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Perspectives in Plant Abiotic Stress Signaling.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Duckweed: a potential phytosensor for heavy metals.Plant cell reports · 2022Review
- Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements.ACS synthetic biology · 2022Article
- Plant Bioelectronics and Biohybrids: The Growing Contribution of Organic Electronic and Carbon-Based Materials.Chemical reviews · 2022Review
- Specific Bacterial Pathogen Phytosensing Is Enabled by a Synthetic Promoter-Transcription Factor System in Potato.Frontiers in plant science · 2022Article
- Performance of abiotic stress-inducible synthetic promoters in genetically engineered hybrid poplar (Frontiers in plant science · 2022Article
- Benchmarking organic electrochemical transistors for plant electrophysiology.Frontiers in plant science · 2022Article
- Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.Horticulture research · 2021Article
- Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants.Plant biotechnology journal · 2021Article
- Generation, analysis, and transformation of macro-chloroplast Potato (Solanum tuberosum) lines for chloroplast biotechnology.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
18 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse genetics approaches have revolutionized plant biology and agriculture. Phenomics has the prospect of bridging plant phenotypes with genes, including transgenes, to transform agricultural fields. Genetically encoded fluorescent proteins (FPs) have revolutionized plant biology paradigms in gene expression, protein trafficking and plant physiology. While the first instance of plant canopy imaging of green fluorescent protein (GFP) was performed over 25 years ago, modern phenomics has largely ignored fluorescence as a transgene expression device despite the burgeoning FP colour palette available to plant biologists. Here, we show a new platform for stand-off imaging of plant canopies expressing a wide variety of FP genes. The platform-the fluorescence-inducing laser projector (FILP)-uses an ultra-low-noise camera to image a scene illuminated by compact diode lasers of various colours, coupled with emission filters to resolve individual FPs, to phenotype transgenic plants expressing FP genes. Each of the 20 FPs screened in plants were imaged at >3 m using FILP in a laboratory-based laser range. We also show that pairs of co-expressed fluorescence proteins can be imaged in canopies. The FILP system enabled a rapid synthetic promoter screen: starting from 2000 synthetic promoters transfected into protoplasts to FILP-imaged agroinfiltrated Nicotiana benthamiana plants in a matter of weeks, which was useful to characterize a water stress-inducible synthetic promoter. FILP canopy imaging was also accomplished for stably transformed GFP potato and in a split-GFP assay, which illustrates the flexibility of the instrument for analysing fluorescence signals in plant canopies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.