ArticleFrontiers in neural circuits2020
Activity-Dependent Remodeling of Synaptic Protein Organization Revealed by High Throughput Analysis of STED Nanoscopy Images.
Article in Frontiers in neural circuits, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED.Methods in microscopy · 2026Article
- A unifying theory of brain signaling and its possible role in acquired chronic disease.Frontiers in neuroscience · 2026Article
- Active Zone: Linking Resolution Levels of Microscopic Modalities.Advances in neurobiology · 2026Review
- Activation of silent synapses driven by emerging technologies: mechanisms, disease associations, and prospects for clinical translation.Frontiers in synaptic neuroscience · 2026Review
- Correlative voltage imaging and cryo-electron tomography bridge neuronal activity and molecular structure.Nature communications · 2025Article
- Neuroplasticity and Nervous System Recovery: Cellular Mechanisms, Therapeutic Advances, and Future Prospects.Brain sciences · 2025Review
- Far-red fluorescent genetically encoded calcium ion indicators.Nature communications · 2025Article
- Sensory experience controls dendritic structure and behavior by distinct pathways involving degenerins.eLife · 2025Article
- Non-canonical function of ADAM10 in presynaptic plasticity.Cellular and molecular life sciences : CMLS · 2024Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Characterizing the blood-brain barrier and gut barrier with super-resolution imaging: opportunities and challenges.Neurophotonics · 2023Review
- GelMap: intrinsic calibration and deformation mapping for expansion microscopy.Nature methods · 2023Article
- Selective TLR4 Antagonism Prevents and Reverses Morphine-Induced Persistent Postoperative Cognitive Dysfunction, Dysregulation of Synaptic Elements, and Impaired BDNF Signaling in Aged Male Rats.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Resolution enhancement with a task-assisted GAN to guide optical nanoscopy image analysis and acquisition.Nature machine intelligence · 2023Article
- Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).eLife · 2022Article
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
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7 authors.
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Abstract
The organization of proteins in the apposed nanodomains of pre- and postsynaptic compartments is thought to play a pivotal role in synaptic strength and plasticity. As such, the alignment between pre- and postsynaptic proteins may regulate, for example, the rate of presynaptic release or the strength of postsynaptic signaling. However, the analysis of these structures has mainly been restricted to subsets of synapses, providing a limited view of the diversity of synaptic protein cluster remodeling during synaptic plasticity. To characterize changes in the organization of synaptic nanodomains during synaptic plasticity over a large population of synapses, we combined STimulated Emission Depletion (STED) nanoscopy with a Python-based statistical object distance analysis (pySODA), in dissociated cultured hippocampal circuits exposed to treatments driving different forms of synaptic plasticity. The nanoscale organization, characterized in terms of coupling properties, of presynaptic (Bassoon, RIM1/2) and postsynaptic (PSD95, Homer1c) scaffold proteins was differently altered in response to plasticity-inducing stimuli. For the Bassoon - PSD95 pair, treatments driving synaptic potentiation caused an increase in their coupling probability, whereas a stimulus driving synaptic depression had an opposite effect. To enrich the characterization of the synaptic cluster remodeling at the population level, we applied unsupervised machine learning approaches to include selected morphological features into a multidimensional analysis. This combined analysis revealed a large diversity of synaptic protein cluster subtypes exhibiting differential activity-dependent remodeling, yet with common features depending on the expected direction of plasticity. The expanded palette of synaptic features revealed by our unbiased approach should provide a basis to further explore the widely diverse molecular mechanisms of synaptic plasticity.
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