Evidence map›Paper›PMID 33177605›Full record

ArticleScientific reports2020

Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging.

Kazuki Harada, Takami Chihara, Yuki Hayasaka, Marie Mita, Mai Takizawa, Kentaro Ishida, Mary Arai, Saki Tsuno, Mitsuharu Matsumoto, Takeshi Ishihara and 3 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 44 citations in OpenAlex.

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  17. Electro-metabolic signaling.The Journal of general physiology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Kazuki HaradaDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan.
Takami ChiharaDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-0033, Japan.
Yuki HayasakaDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan.
Marie MitaDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan.
Mai TakizawaDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan.
Kentaro IshidaMyoridge Co. Ltd., 46-29 Yoshidashimoadachi-cho, Sakyo, Kyoto, 606-8501, Japan.
Mary AraiDepartment of Biology, Faculty of Science, Kyushu University, 774 Motooka, Nishi, Fukuoka, 819-0395, Japan.
Saki TsunoDairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd., Hinode-machi, Nishitama-gun, Tokyo, 190-0182, Japan.
Mitsuharu MatsumotoDairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd., Hinode-machi, Nishitama-gun, Tokyo, 190-0182, Japan.
Takeshi IshiharaDepartment of Biology, Faculty of Science, Kyushu University, 774 Motooka, Nishi, Fukuoka, 819-0395, Japan.
Hiroshi UedaLaboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8503, Japan.
Tetsuya KitaguchiLaboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8503, Japan. kitaguct-gfp@umin.ac.jp.
Takashi TsuboiDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan. takatsuboi@bio.c.u-tokyo.ac.jp.
The University of Tokyo · JPKyushu University · JPLife Science Institute · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glycolysis is the metabolic pathway that converts glucose into pyruvate, whereas fermentation can then produce lactate from pyruvate. Here, we developed single fluorescent protein (FP)-based lactate and pyruvate indicators with low EC

Indexed as

AnimalsBiosensing TechniquesCells, CulturedFemaleGlycolysisGreen Fluorescent ProteinsHeLa CellsHumansLactic AcidMice, Inbred ICRMolecular ImagingMyocytes, CardiacNeuronsOligomycinsPregnancyPyruvic AcidGreen Fluorescent ProteinsLactic AcidOligomycinsPyruvic AcidRecombinant ProteinsSerine

Identifiers

PMID33177605
PMCPMC7659002
OpenAlexW3105424763

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.